Quantitative analysis of neural stem cell growth and differentiation
Neural stem cells (NSCs) are self-renewing, multipotent cells in the embryonic or adult brain that can give rise to neurons and glia. NSCs and neural progenitors (NPs) can be propagated in vitro through a natural 3-D culture system known as neurospheres. Here, I report the use of non-invasive image...
Saved in:
Main Author: | |
---|---|
Other Authors: | |
Format: | Theses and Dissertations |
Language: | English |
Published: |
2014
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/55362 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
Summary: | Neural stem cells (NSCs) are self-renewing, multipotent cells in the embryonic or adult brain that can give rise to neurons and glia. NSCs and neural progenitors (NPs) can be propagated in vitro through a natural 3-D culture system known as neurospheres. Here, I report the use of non-invasive image acquisition and processing techniques to gain insight into the 3-D architecture of the neurosphere, characterise neurosphere forming cells (NFCs), develop an imaging-based method to enrich for NSCs, and a high throughput screening (HTS) strategy to study the effects of drugs on NSCs/NPs. |
---|