Quantitative analysis of neural stem cell growth and differentiation
Neural stem cells (NSCs) are self-renewing, multipotent cells in the embryonic or adult brain that can give rise to neurons and glia. NSCs and neural progenitors (NPs) can be propagated in vitro through a natural 3-D culture system known as neurospheres. Here, I report the use of non-invasive image...
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sg-ntu-dr.10356-553622023-02-28T18:45:43Z Quantitative analysis of neural stem cell growth and differentiation Shvetha Sankaran Sohail Ahmed Koh Cheng Gee School of Biological Sciences A*STAR Institute of Medical Biology DRNTU::Science::Biological sciences::Cytology Neural stem cells (NSCs) are self-renewing, multipotent cells in the embryonic or adult brain that can give rise to neurons and glia. NSCs and neural progenitors (NPs) can be propagated in vitro through a natural 3-D culture system known as neurospheres. Here, I report the use of non-invasive image acquisition and processing techniques to gain insight into the 3-D architecture of the neurosphere, characterise neurosphere forming cells (NFCs), develop an imaging-based method to enrich for NSCs, and a high throughput screening (HTS) strategy to study the effects of drugs on NSCs/NPs. DOCTOR OF PHILOSOPHY (SBS) 2014-02-17T06:31:05Z 2014-02-17T06:31:05Z 2014 2014 Thesis Shvetha Sankaran. (2014). Quantitative analysis of neural stem cell growth and differentiation. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/55362 10.32657/10356/55362 en 161 p. application/pdf |
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DRNTU::Science::Biological sciences::Cytology Shvetha Sankaran Quantitative analysis of neural stem cell growth and differentiation |
| description |
Neural stem cells (NSCs) are self-renewing, multipotent cells in the embryonic or adult brain that can give rise to neurons and glia. NSCs and neural progenitors (NPs) can be propagated in vitro through a natural 3-D culture system known as neurospheres. Here, I report the use of non-invasive image acquisition and processing techniques to gain insight into the 3-D architecture of the neurosphere, characterise neurosphere forming cells (NFCs), develop an imaging-based method to enrich for NSCs, and a high throughput screening (HTS) strategy to study the effects of drugs on NSCs/NPs. |
| author2 |
Sohail Ahmed |
| author_facet |
Sohail Ahmed Shvetha Sankaran |
| format |
Theses and Dissertations |
| author |
Shvetha Sankaran |
| author_sort |
Shvetha Sankaran |
| title |
Quantitative analysis of neural stem cell growth and differentiation |
| title_short |
Quantitative analysis of neural stem cell growth and differentiation |
| title_full |
Quantitative analysis of neural stem cell growth and differentiation |
| title_fullStr |
Quantitative analysis of neural stem cell growth and differentiation |
| title_full_unstemmed |
Quantitative analysis of neural stem cell growth and differentiation |
| title_sort |
quantitative analysis of neural stem cell growth and differentiation |
| publishDate |
2014 |
| url |
https://hdl.handle.net/10356/55362 |
| _version_ |
1759856831559106560 |