Structural and functional insights into subunit a of Saccharomyces cerevisiae V-ATPase and the Escherichia coli Alkyl Hydroperoxide Reductase complex
The new function of subunit a of the V1VO ATPase as pH sensing receptor and its regulatory binding sites, a21-17 and a2368-395 to the Sec7 domain of ARNO (ADP-ribosylation factor Nucleotide site Opener) were studied using NMR spectroscopy. The results of NMR titration experiments of mouse a21-17 and...
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sg-ntu-dr.10356-553952023-02-28T18:35:12Z Structural and functional insights into subunit a of Saccharomyces cerevisiae V-ATPase and the Escherichia coli Alkyl Hydroperoxide Reductase complex Phat Vinh, Dip Gerhard Gruber School of Biological Sciences DRNTU::Science::Biological sciences::Biochemistry The new function of subunit a of the V1VO ATPase as pH sensing receptor and its regulatory binding sites, a21-17 and a2368-395 to the Sec7 domain of ARNO (ADP-ribosylation factor Nucleotide site Opener) were studied using NMR spectroscopy. The results of NMR titration experiments of mouse a21-17 and yeast a104-363 and small angle X-ray scattering (SAXS) of a104-363 suggested a new molecular mechanism between V-ATPase and ARNO in proton-pumping activity and vesicle formation. Besides pH homeostasis, regulated via V-ATPases, redox homeostasis is primary balanced via the Alkyl Hydroperoxide Reductase (AhpR). The crystal structure of both AhpR subunits, the 56 kDa subunit F (AhpF) and 21 kDa subunit C (AhpC) from Escherichia coli have been solved to 2 Å and 3.3 Å resolution. Together with AhpF SAXS analysis and cryo electron microscopy (cryoEM) studies of AhpC, the catalytic mechanism of AhpR as hydroperoxide scavenger and molecular chaperon are described in this thesis. DOCTOR OF PHILOSOPHY (SBS) 2014-02-26T03:31:11Z 2014-02-26T03:31:11Z 2014 2014 Thesis Phat Vinh, D. (2014). Structural and functional insights into subunit a of Saccharomyces cerevisiae V-ATPase and the Escherichia coli Alkyl Hydroperoxide Reductase complex. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/55395 10.32657/10356/55395 en 217 p. application/pdf |
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DRNTU::Science::Biological sciences::Biochemistry Phat Vinh, Dip Structural and functional insights into subunit a of Saccharomyces cerevisiae V-ATPase and the Escherichia coli Alkyl Hydroperoxide Reductase complex |
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The new function of subunit a of the V1VO ATPase as pH sensing receptor and its regulatory binding sites, a21-17 and a2368-395 to the Sec7 domain of ARNO (ADP-ribosylation factor Nucleotide site Opener) were studied using NMR spectroscopy. The results of NMR titration experiments of mouse a21-17 and yeast a104-363 and small angle X-ray scattering (SAXS) of a104-363 suggested a new molecular mechanism between V-ATPase and ARNO in proton-pumping activity and vesicle formation. Besides pH homeostasis, regulated via V-ATPases, redox homeostasis is primary balanced via the Alkyl Hydroperoxide Reductase (AhpR). The crystal structure of both AhpR subunits, the 56 kDa subunit F (AhpF) and 21 kDa subunit C (AhpC) from Escherichia coli have been solved to 2 Å and 3.3 Å resolution. Together with AhpF SAXS analysis and cryo electron microscopy (cryoEM) studies of AhpC, the catalytic mechanism of AhpR as hydroperoxide scavenger and molecular chaperon are described in this thesis. |
author2 |
Gerhard Gruber |
author_facet |
Gerhard Gruber Phat Vinh, Dip |
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Theses and Dissertations |
author |
Phat Vinh, Dip |
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Phat Vinh, Dip |
title |
Structural and functional insights into subunit a of Saccharomyces cerevisiae V-ATPase and the Escherichia coli Alkyl Hydroperoxide Reductase complex |
title_short |
Structural and functional insights into subunit a of Saccharomyces cerevisiae V-ATPase and the Escherichia coli Alkyl Hydroperoxide Reductase complex |
title_full |
Structural and functional insights into subunit a of Saccharomyces cerevisiae V-ATPase and the Escherichia coli Alkyl Hydroperoxide Reductase complex |
title_fullStr |
Structural and functional insights into subunit a of Saccharomyces cerevisiae V-ATPase and the Escherichia coli Alkyl Hydroperoxide Reductase complex |
title_full_unstemmed |
Structural and functional insights into subunit a of Saccharomyces cerevisiae V-ATPase and the Escherichia coli Alkyl Hydroperoxide Reductase complex |
title_sort |
structural and functional insights into subunit a of saccharomyces cerevisiae v-atpase and the escherichia coli alkyl hydroperoxide reductase complex |
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2014 |
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https://hdl.handle.net/10356/55395 |
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1759854121494511616 |