Alternative cleavage and polyadenylation in human CD46
Although well-known as a negative regulator of complement activation, human transmembrane protein CD46 plays multiple roles in immunity and immune-independent processes. Various mechanisms, including alternative splicing and posttranslational modifications, contribute to the CD46’s multitasking thro...
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sg-ntu-dr.10356-597662023-02-28T18:00:37Z Alternative cleavage and polyadenylation in human CD46 Ly, Phuong Thao School of Biological Sciences Francesc Xavier Roca Castella DRNTU::Science Although well-known as a negative regulator of complement activation, human transmembrane protein CD46 plays multiple roles in immunity and immune-independent processes. Various mechanisms, including alternative splicing and posttranslational modifications, contribute to the CD46’s multitasking through generating isoforms with distinct functions. However, the role of Alternative Polyadenylation (APA), by which a gene creates various transcripts different either in their 3’ untranslated region (3’UTR) or Open Reading Frame (ORF), is yet unestablished for CD46. In this study, we aimed to characterize APA in CD46, focusing on APA occurring in introns or the 3’ terminal exon. To achieve this goal, we exploited the properties of APA-generated mRNAs such as intronic retention in mRNAs, the responsiveness to functional U1 levels of intronically terminated transcripts, and we performed 3’RACE to detect alternatively polyadenylated mRNAs. By the end of this study, we have successfully demonstrated that CD46 undergoes multiple types of APA, by which various truncated mRNAs altered either in their ORFs or 3’UTRs are generated. These results ultimately underscore the potential of APA as an essential mechanism to regulate CD46 expression and function. Bachelor of Science in Biological Sciences 2014-05-14T04:56:46Z 2014-05-14T04:56:46Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/59766 en Nanyang Technological University 37 p. application/pdf |
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Although well-known as a negative regulator of complement activation, human transmembrane protein CD46 plays multiple roles in immunity and immune-independent processes. Various mechanisms, including alternative splicing and posttranslational modifications, contribute to the CD46’s multitasking through generating isoforms with distinct functions. However, the role of Alternative Polyadenylation (APA), by which a gene creates various transcripts different either in their 3’ untranslated region (3’UTR) or Open Reading Frame (ORF), is yet unestablished for CD46. In this study, we aimed to characterize APA in CD46, focusing on APA occurring in introns or the 3’ terminal exon. To achieve this goal, we exploited the properties of APA-generated mRNAs such as intronic retention in mRNAs, the responsiveness to functional U1 levels of intronically terminated transcripts, and we performed 3’RACE to detect alternatively polyadenylated mRNAs. By the end of this study, we have successfully demonstrated that CD46 undergoes multiple types of APA, by which various truncated mRNAs altered either in their ORFs or 3’UTRs are generated. These results ultimately underscore the potential of APA as an essential mechanism to regulate CD46 expression and function. |
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School of Biological Sciences |
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School of Biological Sciences Ly, Phuong Thao |
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Final Year Project |
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Ly, Phuong Thao |
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Ly, Phuong Thao |
title |
Alternative cleavage and polyadenylation in human CD46 |
title_short |
Alternative cleavage and polyadenylation in human CD46 |
title_full |
Alternative cleavage and polyadenylation in human CD46 |
title_fullStr |
Alternative cleavage and polyadenylation in human CD46 |
title_full_unstemmed |
Alternative cleavage and polyadenylation in human CD46 |
title_sort |
alternative cleavage and polyadenylation in human cd46 |
publishDate |
2014 |
url |
http://hdl.handle.net/10356/59766 |
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1759855187114065920 |