Metabolomics analysis for quorum sensing biomarkers in Pseudomonas aeruginosa biofilms

Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of medical problems and can be difficult to eradicate by conventional antimicrobials due to formation of biofilms. Quorum sensing, the process by which bacteria communicate with each other through the use of chemical signal...

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Main Author: Chen, Junzheng
Other Authors: Yang Liang
Format: Final Year Project
Language:English
Published: 2014
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Online Access:http://hdl.handle.net/10356/60723
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-607232023-02-28T18:06:45Z Metabolomics analysis for quorum sensing biomarkers in Pseudomonas aeruginosa biofilms Chen, Junzheng Yang Liang School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences::Microbiology::Bacteria Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of medical problems and can be difficult to eradicate by conventional antimicrobials due to formation of biofilms. Quorum sensing, the process by which bacteria communicate with each other through the use of chemical signals, plays a pivotal role in biofilm formation. Through a metabolomics approach, we aim to study the metabolic processes of wild-type P. aeruginosa PAO1 strain and its relationship to biofilm formation, and also discover biomarkers which could be used for biofilm detection. From our chemostat setup, the expression of QS signals from rhlA, lasB and pqs reporter genes were reportedly the highest at 0.03 dilution rate. A comparison between NMR spectra shows that rhamnolipid and 2-heptyl-4-quinolone were found to be higher in a nutrient limited environment (ABTG media). By matching the NMR peaks with online databases (MMCD and KEGG), we have identified potential biomarkers related to Pseudomonas Quinolone Signal mediated quorum sensing and isoleucine synthesis pathway. Using LCMS analysis as a complement, D-erythro-3-Methylmalate was identified as a precursor of isoleucine. This demonstrates metabolomics is a flexible and systemic approach towards understanding metabolic network of quorum sensing mechanism and discovery of biomarkers. Bachelor of Science in Biological Sciences 2014-05-29T07:26:46Z 2014-05-29T07:26:46Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/60723 en Nanyang Technological University 56 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology::Bacteria
spellingShingle DRNTU::Science::Biological sciences::Microbiology::Bacteria
Chen, Junzheng
Metabolomics analysis for quorum sensing biomarkers in Pseudomonas aeruginosa biofilms
description Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of medical problems and can be difficult to eradicate by conventional antimicrobials due to formation of biofilms. Quorum sensing, the process by which bacteria communicate with each other through the use of chemical signals, plays a pivotal role in biofilm formation. Through a metabolomics approach, we aim to study the metabolic processes of wild-type P. aeruginosa PAO1 strain and its relationship to biofilm formation, and also discover biomarkers which could be used for biofilm detection. From our chemostat setup, the expression of QS signals from rhlA, lasB and pqs reporter genes were reportedly the highest at 0.03 dilution rate. A comparison between NMR spectra shows that rhamnolipid and 2-heptyl-4-quinolone were found to be higher in a nutrient limited environment (ABTG media). By matching the NMR peaks with online databases (MMCD and KEGG), we have identified potential biomarkers related to Pseudomonas Quinolone Signal mediated quorum sensing and isoleucine synthesis pathway. Using LCMS analysis as a complement, D-erythro-3-Methylmalate was identified as a precursor of isoleucine. This demonstrates metabolomics is a flexible and systemic approach towards understanding metabolic network of quorum sensing mechanism and discovery of biomarkers.
author2 Yang Liang
author_facet Yang Liang
Chen, Junzheng
format Final Year Project
author Chen, Junzheng
author_sort Chen, Junzheng
title Metabolomics analysis for quorum sensing biomarkers in Pseudomonas aeruginosa biofilms
title_short Metabolomics analysis for quorum sensing biomarkers in Pseudomonas aeruginosa biofilms
title_full Metabolomics analysis for quorum sensing biomarkers in Pseudomonas aeruginosa biofilms
title_fullStr Metabolomics analysis for quorum sensing biomarkers in Pseudomonas aeruginosa biofilms
title_full_unstemmed Metabolomics analysis for quorum sensing biomarkers in Pseudomonas aeruginosa biofilms
title_sort metabolomics analysis for quorum sensing biomarkers in pseudomonas aeruginosa biofilms
publishDate 2014
url http://hdl.handle.net/10356/60723
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