Signals and cues driving intraspecific variation in mixed community biofilms
Biofilms are surface-associated bacteria that can occur as mixed-species communities. Synergistic interactions between members of a Pseudomonas aeruginosa, Pseudomonas protegens and Klebsiella pneumoniae when grown as a mixed-species biofilm have been discovered to reduce intraspecies genetic variat...
Saved in:
| 主要作者: | |
|---|---|
| 其他作者: | |
| 格式: | Final Year Project |
| 語言: | English |
| 出版: |
2015
|
| 主題: | |
| 在線閱讀: | http://hdl.handle.net/10356/63464 |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|
| 機構: | Nanyang Technological University |
| 語言: | English |
| 總結: | Biofilms are surface-associated bacteria that can occur as mixed-species communities. Synergistic interactions between members of a Pseudomonas aeruginosa, Pseudomonas protegens and Klebsiella pneumoniae when grown as a mixed-species biofilm have been discovered to reduce intraspecies genetic variation in all three species via both extracellular signals and genetic regulatory pathways. The work done in this project determined that the extracellular signals involved in Pseudomonas protegens genetic variant suppression could not be extracted using standard methods to isolate non-polar compounds. Therefore an extraction protocol targeting polar metabolites was developed using acetonitrile combined with either sodium sulphate or ammonium sulphate. Using this method, it was possible to extract metabolites from K. pneumoniae mono-species biofilm effluent that suppressed variant formation when added back to P. protegens biofilms. This provides a method for subsequent fractionation and identification of active compounds involved in the process. The project also proposed that the recombinase A gene, recA which is involved in DNA repair and stress responses may also play a role in variant suppression. Therefore a gene deletion strategy was devised to knockout recA in P. aeruginosa and P. protegens. |
|---|