Investigation of drug targets for pseudomonas aeruginosa

The increased prevalence of multi-drug resistant Pseudomonas aeruginosa and inadequacy of conventional antimicrobial solutions have been a major concern worldwide. Hence, there is an urgent need of identifying novel drug targets. In this study, artificial sputum medium(ASM) and M9 supplemented media...

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Main Author: Tan, Alvin Swee Leong
Other Authors: Kevin Pethe
Format: Final Year Project
Language:English
Published: 2015
Subjects:
Online Access:http://hdl.handle.net/10356/65634
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-656342023-02-28T17:59:45Z Investigation of drug targets for pseudomonas aeruginosa Tan, Alvin Swee Leong Kevin Pethe School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology The increased prevalence of multi-drug resistant Pseudomonas aeruginosa and inadequacy of conventional antimicrobial solutions have been a major concern worldwide. Hence, there is an urgent need of identifying novel drug targets. In this study, artificial sputum medium(ASM) and M9 supplemented media(M9SM) were used to study P. aeruginosa strain PAO1 growth and drug compound testing. ASM have been used as the culture medium as it bears close resemblance to the environment of the lungs in cystic fibrosis patients. Potency of drug compound oxythiamine was influenced by manipulation of components in growth media. Casamino acid have been found to reduce oxythiamine potency. As oxythiamine affects a variety of other thiamin pyrophosphate(TPP)-dependent enzymes, its ability to display synergistic effect when used in combination with other compounds is also examined. Fractional inhibitory assay have shown synergistic combinatorial effect between oxythiamine coupled with either oxytetracycline or 5-fluorouracil. Combination of oxythiamine and patulin however, reflects combinatorial effect which is slightly antagonistic. The information gathered from this study would provide a basis for further transcriptomic studies and identification of potential drug targets in P. aeruginosa. Bachelor of Science in Biological Sciences 2015-11-26T02:34:45Z 2015-11-26T02:34:45Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/65634 en Nanyang Technological University 25 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology
spellingShingle DRNTU::Science::Biological sciences::Microbiology
Tan, Alvin Swee Leong
Investigation of drug targets for pseudomonas aeruginosa
description The increased prevalence of multi-drug resistant Pseudomonas aeruginosa and inadequacy of conventional antimicrobial solutions have been a major concern worldwide. Hence, there is an urgent need of identifying novel drug targets. In this study, artificial sputum medium(ASM) and M9 supplemented media(M9SM) were used to study P. aeruginosa strain PAO1 growth and drug compound testing. ASM have been used as the culture medium as it bears close resemblance to the environment of the lungs in cystic fibrosis patients. Potency of drug compound oxythiamine was influenced by manipulation of components in growth media. Casamino acid have been found to reduce oxythiamine potency. As oxythiamine affects a variety of other thiamin pyrophosphate(TPP)-dependent enzymes, its ability to display synergistic effect when used in combination with other compounds is also examined. Fractional inhibitory assay have shown synergistic combinatorial effect between oxythiamine coupled with either oxytetracycline or 5-fluorouracil. Combination of oxythiamine and patulin however, reflects combinatorial effect which is slightly antagonistic. The information gathered from this study would provide a basis for further transcriptomic studies and identification of potential drug targets in P. aeruginosa.
author2 Kevin Pethe
author_facet Kevin Pethe
Tan, Alvin Swee Leong
format Final Year Project
author Tan, Alvin Swee Leong
author_sort Tan, Alvin Swee Leong
title Investigation of drug targets for pseudomonas aeruginosa
title_short Investigation of drug targets for pseudomonas aeruginosa
title_full Investigation of drug targets for pseudomonas aeruginosa
title_fullStr Investigation of drug targets for pseudomonas aeruginosa
title_full_unstemmed Investigation of drug targets for pseudomonas aeruginosa
title_sort investigation of drug targets for pseudomonas aeruginosa
publishDate 2015
url http://hdl.handle.net/10356/65634
_version_ 1759854678736109568