Chemical and enzymatic methods for selective peptide and protein modification or synthesis

Because almost all peptides and proteins have only one N- and one C-terminus, modification targeting terminal residues is obviously an attractive way to introduce a functional group into a peptide or a protein at a single site as compared to the reactions on the amino acid side chains. This forms th...

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Main Author: Tan, Xiaohong
Other Authors: Liu Chuan Fa
Format: Theses and Dissertations
Published: 2008
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Online Access:https://hdl.handle.net/10356/6578
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-65782020-12-02T01:33:58Z Chemical and enzymatic methods for selective peptide and protein modification or synthesis Tan, Xiaohong Liu Chuan Fa School of Biological Sciences DRNTU::Science::Biological sciences Because almost all peptides and proteins have only one N- and one C-terminus, modification targeting terminal residues is obviously an attractive way to introduce a functional group into a peptide or a protein at a single site as compared to the reactions on the amino acid side chains. This forms the rationale of our study. First, I aim to provide reliable information about the transamination reaction to make future use of this NT-specific protein modification reaction in a much more predictable way. Second, on the peptide CT-modification aspect, this study aims to develop new chemical and enzymatic methods to synthesize peptidyl thioesters and thioacids which are important derivatives to introduce new functional groups onto the C-termini of proteins and peptides. DOCTOR OF PHILOSOPHY (SBS) 2008-09-17T11:42:09Z 2008-09-17T11:42:09Z 2008 2008 Thesis Tan, X. H. (2008). Chemical and enzymatic methods for selective peptide and protein modification or synthesis. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/6578 10.32657/10356/6578 Nanyang Technological University application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Tan, Xiaohong
Chemical and enzymatic methods for selective peptide and protein modification or synthesis
description Because almost all peptides and proteins have only one N- and one C-terminus, modification targeting terminal residues is obviously an attractive way to introduce a functional group into a peptide or a protein at a single site as compared to the reactions on the amino acid side chains. This forms the rationale of our study. First, I aim to provide reliable information about the transamination reaction to make future use of this NT-specific protein modification reaction in a much more predictable way. Second, on the peptide CT-modification aspect, this study aims to develop new chemical and enzymatic methods to synthesize peptidyl thioesters and thioacids which are important derivatives to introduce new functional groups onto the C-termini of proteins and peptides.
author2 Liu Chuan Fa
author_facet Liu Chuan Fa
Tan, Xiaohong
format Theses and Dissertations
author Tan, Xiaohong
author_sort Tan, Xiaohong
title Chemical and enzymatic methods for selective peptide and protein modification or synthesis
title_short Chemical and enzymatic methods for selective peptide and protein modification or synthesis
title_full Chemical and enzymatic methods for selective peptide and protein modification or synthesis
title_fullStr Chemical and enzymatic methods for selective peptide and protein modification or synthesis
title_full_unstemmed Chemical and enzymatic methods for selective peptide and protein modification or synthesis
title_sort chemical and enzymatic methods for selective peptide and protein modification or synthesis
publishDate 2008
url https://hdl.handle.net/10356/6578
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