Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment

Drug-loaded microspheres are widely used in cancer treatment through targeted and sustained drug delivery. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with paclitaxel (PTX) are synthesized and size-fractionated using a novel inertial microfluidics strategy (Dean Flow Frac...

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Main Author: Tan, Kah Kee
Other Authors: Loo Say Chye Joachim
Format: Final Year Project
Language:English
Published: 2016
Subjects:
Online Access:http://hdl.handle.net/10356/66444
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-664442023-03-04T15:41:13Z Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment Tan, Kah Kee Loo Say Chye Joachim School of Materials Science and Engineering DRNTU::Engineering Drug-loaded microspheres are widely used in cancer treatment through targeted and sustained drug delivery. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with paclitaxel (PTX) are synthesized and size-fractionated using a novel inertial microfluidics strategy (Dean Flow Fractionation) for enhanced inhibition of breast cancer cell proliferation. The polydimethylsiloxane (PDMS) microdevice was characterized by optical imaging methods (high speed and fluorescence) and flow cytometry using fluorescent microbeads. After device optimization, PTX-loaded microparticles were fractionated into 3 different size groups (>5µm, 3-5µm and <3µm) and co-incubated with MCF-7 breast cancer cells. CCK Proliferation (CCK) assay and imaging showed that smaller microparticles (<3µm) were more effective at inhibiting cell proliferation. The developed technology provides a novel high throughput sample preparation method for nanoparticle synthesis and drug delivery systems. Bachelor of Engineering (Materials Engineering) 2016-04-07T04:45:04Z 2016-04-07T04:45:04Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/66444 en Nanyang Technological University 36 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering
spellingShingle DRNTU::Engineering
Tan, Kah Kee
Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment
description Drug-loaded microspheres are widely used in cancer treatment through targeted and sustained drug delivery. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with paclitaxel (PTX) are synthesized and size-fractionated using a novel inertial microfluidics strategy (Dean Flow Fractionation) for enhanced inhibition of breast cancer cell proliferation. The polydimethylsiloxane (PDMS) microdevice was characterized by optical imaging methods (high speed and fluorescence) and flow cytometry using fluorescent microbeads. After device optimization, PTX-loaded microparticles were fractionated into 3 different size groups (>5µm, 3-5µm and <3µm) and co-incubated with MCF-7 breast cancer cells. CCK Proliferation (CCK) assay and imaging showed that smaller microparticles (<3µm) were more effective at inhibiting cell proliferation. The developed technology provides a novel high throughput sample preparation method for nanoparticle synthesis and drug delivery systems.
author2 Loo Say Chye Joachim
author_facet Loo Say Chye Joachim
Tan, Kah Kee
format Final Year Project
author Tan, Kah Kee
author_sort Tan, Kah Kee
title Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment
title_short Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment
title_full Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment
title_fullStr Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment
title_full_unstemmed Microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment
title_sort microfluidics size fractionation of paclitaxel-loaded microspheres for breast cancer treatment
publishDate 2016
url http://hdl.handle.net/10356/66444
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