Development of UV-crosslinkable hybrid 3D hydrogels
The project aims to develop UV-crosslinkable hybrid 3D hydrogel with tailorable physical and chemical properties for future cell culture studies in the field of tissue engineering. A hydrogel system were designed by incorporating FN910 protein into a PEG precursor. The protein were expressed and pur...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Final Year Project |
| Language: | English |
| Published: |
2016
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/66477 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | The project aims to develop UV-crosslinkable hybrid 3D hydrogel with tailorable physical and chemical properties for future cell culture studies in the field of tissue engineering. A hydrogel system were designed by incorporating FN910 protein into a PEG precursor. The protein were expressed and purified successfully, and were subsequently crosslinked into the PEG via UV-initiated thiol-ene click chemistry. The resulting hydrogel were characterized by varying the ratio of the protein and the PEG. The hydrogel system was shown to be tunable into a wide range of stiffness, demonstrating their promise in imitating natural body tissues, while being independent from biochemical cues. |
|---|