In vitro sustained release of novel natriuretic peptide from solvent evaporation delivery system
Solvent evaporation technique has been widely studied and is an established method for producing biodegradable Nano or micro particles for pharmaceutical applications. Biodegradable polymers such as poly (lactic-co-glycolic acid) (PLGA) is commonly used as drug carriers due to its biodegradabi...
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Format: | Final Year Project |
Language: | English |
Published: |
2016
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Online Access: | http://hdl.handle.net/10356/66505 |
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Institution: | Nanyang Technological University |
Language: | English |
Summary: | Solvent evaporation technique has been widely studied and is an established
method for producing biodegradable Nano or micro particles for pharmaceutical
applications. Biodegradable polymers such as poly (lactic-co-glycolic acid) (PLGA) is
commonly used as drug carriers due to its biodegradability without any toxic by
products, which can be tailored for use as sustained drug delivery systems.
However, the hydrophobic nature of PLGA makes it difficult to entrap hydrophilic
drugs such as peptides to a good extent. This could pose problems for sustained
drug delivery due to insufficient amount of drug available to work therapeutically or
short release time frames which could be inefficacious. Hence in this project, the
effects of additives on drug loading and loading efficiency are investigated.
Studies were conducted based on two types of additives added to the polymer and
drug solution during fabrication via solvent evaporation technique. The additives
are PEG (5000)-PCL (10000) and Pluronic F127, of which both are amphiphilic in
nature. The idea was to use this property to improve hydrophilic drug encapsulation
into the hydrophobic polymer. One of the fabrication conditions, the oil phase
(PLGA in DCM) to aqueous phase (drug in 1% PVA) ratio, was also changed such that
both Nano and micro particles were produced. The effects of the additives on the
size of the particles were also studied.
Release study results show that Pluronic F127 had a better effect in improving the
drug loading and loading efficiency for micro particles. There was an almost three
fold improvement in loading efficiency compared to adding F127 to the
nanoparticles, due to an increase in the overall hydrophilicity of the particle. The
increase in hydrophilicity decreased the solubility of the polymer in the organic
solvent and facilitated faster polymer solidification, which was able to encapsulate
more drugs. |
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