In vitro reconstitution of telomeric mono-nucleosomes

• المؤلف الرئيسي: Tiu, Charles Kevin Dee
• مؤلفون آخرون: Lars Nordenskiold
• التنسيق: Final Year Project
• اللغة: English
• منشور في: 2016
• الموضوعات: DRNTU::Science::Biological sciences::Molecular biology; DRNTU::Science::Biological sciences::Biophysics
• الوصول للمادة أونلاين: http://hdl.handle.net/10356/67347

Reconstitution of telomeric DNA (telo-DNA) with human histone octamer, to form telomeric nucleosomes, was often quoted as challenging given that telo-DNA does not possess a positioning sequence and is out-of-sync with the helical periodicity of DNA. In this report, I endeavor to demonstrate telomeri...