Investigating the effect of betamethasone loaded lipid-polymer hybrid nanoparticles for autoimmune diseases

Presently, the use of nanoparticles (NP) to encapsulate and deliver drugs for treatment of autoimmune diseases such as psoriasis and rheumatoid arthritis focus on targeting macrophages to reduce inflammatory response. Tumor necrosis factor alpha (TNF-α) is expressed by macrophages during inflammatio...

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Main Author: Su, Isabella Jing Tian
Other Authors: Chan Shuping Juliana Maria
Format: Final Year Project
Language:English
Published: 2016
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Online Access:http://hdl.handle.net/10356/68405
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-684052023-03-03T15:36:19Z Investigating the effect of betamethasone loaded lipid-polymer hybrid nanoparticles for autoimmune diseases Su, Isabella Jing Tian Chan Shuping Juliana Maria School of Chemical and Biomedical Engineering DRNTU::Engineering Presently, the use of nanoparticles (NP) to encapsulate and deliver drugs for treatment of autoimmune diseases such as psoriasis and rheumatoid arthritis focus on targeting macrophages to reduce inflammatory response. Tumor necrosis factor alpha (TNF-α) is expressed by macrophages during inflammation and can be used to evaluate the effectiveness of inflammatory drug therapies in reducing TNF-α expression. Betamethasone 17-valerate (B17V) is a corticosteroid commonly used in anti-inflammatory drug formulations to suppress inflammation and the immune system. Using a modified nanoprecipitation method, lipid-polymer hybrid nanoparticles (LPN) were synthesized at 20% wt/wt lipid/polymer mass ratio and 7:3 lipid/lipid-PEG molar ratio. Characterization by dynamic light scattering (DLS) and high performance liquid chromatography (HPLC) identified the optimal drug loaded to be 20% wt/wt. Stability tests were carried out over 120hrs and BLPNs were evaluated to be stable with no significant change in size and polydispersity index over the time period. In vitro treatment of free drug and betamethasone LPN (BLPN) on macrophages showed significant reduction of TNF-α expression from 54.32 pg/ml to 16.95±2.82pg/ml and 20.66±1.63pg/ml respectively, suggesting that BLPNs may be useful as a drug delivery system to reduce inflammatory responses in autoimmune diseases. Treatment with free B17V or B17V-loaded nanoparticles did not show any significant cytotoxicity on the tested monocytes and macrophages. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2016-05-25T09:07:51Z 2016-05-25T09:07:51Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/68405 en Nanyang Technological University 54 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering
spellingShingle DRNTU::Engineering
Su, Isabella Jing Tian
Investigating the effect of betamethasone loaded lipid-polymer hybrid nanoparticles for autoimmune diseases
description Presently, the use of nanoparticles (NP) to encapsulate and deliver drugs for treatment of autoimmune diseases such as psoriasis and rheumatoid arthritis focus on targeting macrophages to reduce inflammatory response. Tumor necrosis factor alpha (TNF-α) is expressed by macrophages during inflammation and can be used to evaluate the effectiveness of inflammatory drug therapies in reducing TNF-α expression. Betamethasone 17-valerate (B17V) is a corticosteroid commonly used in anti-inflammatory drug formulations to suppress inflammation and the immune system. Using a modified nanoprecipitation method, lipid-polymer hybrid nanoparticles (LPN) were synthesized at 20% wt/wt lipid/polymer mass ratio and 7:3 lipid/lipid-PEG molar ratio. Characterization by dynamic light scattering (DLS) and high performance liquid chromatography (HPLC) identified the optimal drug loaded to be 20% wt/wt. Stability tests were carried out over 120hrs and BLPNs were evaluated to be stable with no significant change in size and polydispersity index over the time period. In vitro treatment of free drug and betamethasone LPN (BLPN) on macrophages showed significant reduction of TNF-α expression from 54.32 pg/ml to 16.95±2.82pg/ml and 20.66±1.63pg/ml respectively, suggesting that BLPNs may be useful as a drug delivery system to reduce inflammatory responses in autoimmune diseases. Treatment with free B17V or B17V-loaded nanoparticles did not show any significant cytotoxicity on the tested monocytes and macrophages.
author2 Chan Shuping Juliana Maria
author_facet Chan Shuping Juliana Maria
Su, Isabella Jing Tian
format Final Year Project
author Su, Isabella Jing Tian
author_sort Su, Isabella Jing Tian
title Investigating the effect of betamethasone loaded lipid-polymer hybrid nanoparticles for autoimmune diseases
title_short Investigating the effect of betamethasone loaded lipid-polymer hybrid nanoparticles for autoimmune diseases
title_full Investigating the effect of betamethasone loaded lipid-polymer hybrid nanoparticles for autoimmune diseases
title_fullStr Investigating the effect of betamethasone loaded lipid-polymer hybrid nanoparticles for autoimmune diseases
title_full_unstemmed Investigating the effect of betamethasone loaded lipid-polymer hybrid nanoparticles for autoimmune diseases
title_sort investigating the effect of betamethasone loaded lipid-polymer hybrid nanoparticles for autoimmune diseases
publishDate 2016
url http://hdl.handle.net/10356/68405
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