Engineering E. coli for the biosynthesis of nanoparticles

• Main Author: Koh, Zhong Ren
• Other Authors: Lim Sierin
• Format: Final Year Project
• Language: English
• Published: 2016
• Subjects: DRNTU::Science::Chemistry::Analytical chemistry::Proteins; DRNTU::Engineering::Bioengineering; DRNTU::Science::Biological sciences::Microbiology::Bacteria; DRNTU::Science::Biological sciences::Genetics
• Online Access: http://hdl.handle.net/10356/68471
• Institution: Nanyang Technological University

With the potential of Archaeoglobus fulgidus ferritin to serve as an MRI contrast agent, the study sought to optimize ferritin and feoB production using arabinose induction and to optimize in vivo iron loading in strains of engineered E. coli. The plasmid pBbE8k was primarily used as a vector for f...