Structural and functional studies of leukemia-associated mutations in human cytosolic 5'-nucleotidase II

NT5C2 relapse-associated mutations, K359Q and R367Q, are correlated with large gains in their enzymatic profiles. Crystal structures obtained in this study provide insights to the allosteric regulation mechanism of the enzyme by the disorder-to-order transition of a regulatory helix, which forms par...

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Main Author: Lim, Pei Yiing
Other Authors: Pär Nordlund
Format: Theses and Dissertations
Language:English
Published: 2016
Subjects:
Online Access:http://hdl.handle.net/10356/69099
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-690992023-02-28T18:31:56Z Structural and functional studies of leukemia-associated mutations in human cytosolic 5'-nucleotidase II Lim, Pei Yiing Pär Nordlund School of Biological Sciences DRNTU::Science NT5C2 relapse-associated mutations, K359Q and R367Q, are correlated with large gains in their enzymatic profiles. Crystal structures obtained in this study provide insights to the allosteric regulation mechanism of the enzyme by the disorder-to-order transition of a regulatory helix, which forms part of the active site. In addition, the mutant enzymes possess an increased basal level of activity and sensitivity to activation by effectors. In this study, the structural aspects of how each mutation potentially gains an advantage over the wild-type protein is revealed, with supporting evidence from biochemical and biophysical assays. ​Master of Science 2016-10-28T08:03:26Z 2016-10-28T08:03:26Z 2016 Thesis http://hdl.handle.net/10356/69099 en 100 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science
spellingShingle DRNTU::Science
Lim, Pei Yiing
Structural and functional studies of leukemia-associated mutations in human cytosolic 5'-nucleotidase II
description NT5C2 relapse-associated mutations, K359Q and R367Q, are correlated with large gains in their enzymatic profiles. Crystal structures obtained in this study provide insights to the allosteric regulation mechanism of the enzyme by the disorder-to-order transition of a regulatory helix, which forms part of the active site. In addition, the mutant enzymes possess an increased basal level of activity and sensitivity to activation by effectors. In this study, the structural aspects of how each mutation potentially gains an advantage over the wild-type protein is revealed, with supporting evidence from biochemical and biophysical assays.
author2 Pär Nordlund
author_facet Pär Nordlund
Lim, Pei Yiing
format Theses and Dissertations
author Lim, Pei Yiing
author_sort Lim, Pei Yiing
title Structural and functional studies of leukemia-associated mutations in human cytosolic 5'-nucleotidase II
title_short Structural and functional studies of leukemia-associated mutations in human cytosolic 5'-nucleotidase II
title_full Structural and functional studies of leukemia-associated mutations in human cytosolic 5'-nucleotidase II
title_fullStr Structural and functional studies of leukemia-associated mutations in human cytosolic 5'-nucleotidase II
title_full_unstemmed Structural and functional studies of leukemia-associated mutations in human cytosolic 5'-nucleotidase II
title_sort structural and functional studies of leukemia-associated mutations in human cytosolic 5'-nucleotidase ii
publishDate 2016
url http://hdl.handle.net/10356/69099
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