Functional insight the role of plasmodium falciparum reticulocyte blinding like protein homologues : RH2B and RH5 during merozoites invasion

The process of Plasmodium merozoites invasion is a complex multi-step process. In understanding the mechanisms leading to successful merozoites invasion, a group of proteins known as Plasmodium falciparum erythrocyte binding ligands as well as Plasmodium falciparum reticulocyte binding - like pro...

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Main Author: Yaw, Aniweh
Other Authors: Peter Rainer Preiser
Format: Theses and Dissertations
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/69419
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-694192023-02-28T18:31:04Z Functional insight the role of plasmodium falciparum reticulocyte blinding like protein homologues : RH2B and RH5 during merozoites invasion Yaw, Aniweh Peter Rainer Preiser School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology The process of Plasmodium merozoites invasion is a complex multi-step process. In understanding the mechanisms leading to successful merozoites invasion, a group of proteins known as Plasmodium falciparum erythrocyte binding ligands as well as Plasmodium falciparum reticulocyte binding - like protein homologues (PfRH) have been shown to be necessary. These proteins have been shown to play a role in sensing the erythrocyte. Of the PfRH family, it is becoming clearer of their ability to affect the secretion of microneme proteins which are necessary to establish the tight junction. In this presentation, the role of PfRH2b during merozoite invasion has studied. Like PfRH1, PfRH2b has been shown to regulate the increase in merozoites Ca2+. This, we have shown to affect the release of EBA175 to the erythrocyte surface. Our data also points to the fact that the secretion of EBA175 to the surface is more prominent when the merozoites are engaged with the RBCs. Furthermore we have provided the detailed processing events that occur in the PfRH2b protein before and during merozoites invasion. Functional insight on RH5-Basigin interaction has been provided. In this, we have shown RH5 to be involved in invasion independent of EBA175 surface expression but functions before tight junction formation. We have provided evidence of RH5-Basigin interaction driving Ca2+ in the RBC leading to the remodelling of the RBC cytoskeletal proteins arrangement. We have confirmed that recombinant RH5 has the ability to induce changes in the RBC cytoskeleton upon binding. This mechanism we propose facilitates the insertion of AMA1-RON complex into the RBC membrane leading to tight junction formation. This knowledge we believe will help in advancing our understanding as well as provide avenue to explore the mechanisms of merozoites invasion DOCTOR OF PHILOSOPHY (SBS) 2016-12-29T03:20:28Z 2016-12-29T03:20:28Z 2016 Thesis Yaw, A. (2016). Functional insight the role of plasmodium falciparum reticulocyte blinding like protein homologues : RH2B and RH5 during merozoites invasion. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/69419 10.32657/10356/69419 en 182 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology
spellingShingle DRNTU::Science::Biological sciences::Microbiology
Yaw, Aniweh
Functional insight the role of plasmodium falciparum reticulocyte blinding like protein homologues : RH2B and RH5 during merozoites invasion
description The process of Plasmodium merozoites invasion is a complex multi-step process. In understanding the mechanisms leading to successful merozoites invasion, a group of proteins known as Plasmodium falciparum erythrocyte binding ligands as well as Plasmodium falciparum reticulocyte binding - like protein homologues (PfRH) have been shown to be necessary. These proteins have been shown to play a role in sensing the erythrocyte. Of the PfRH family, it is becoming clearer of their ability to affect the secretion of microneme proteins which are necessary to establish the tight junction. In this presentation, the role of PfRH2b during merozoite invasion has studied. Like PfRH1, PfRH2b has been shown to regulate the increase in merozoites Ca2+. This, we have shown to affect the release of EBA175 to the erythrocyte surface. Our data also points to the fact that the secretion of EBA175 to the surface is more prominent when the merozoites are engaged with the RBCs. Furthermore we have provided the detailed processing events that occur in the PfRH2b protein before and during merozoites invasion. Functional insight on RH5-Basigin interaction has been provided. In this, we have shown RH5 to be involved in invasion independent of EBA175 surface expression but functions before tight junction formation. We have provided evidence of RH5-Basigin interaction driving Ca2+ in the RBC leading to the remodelling of the RBC cytoskeletal proteins arrangement. We have confirmed that recombinant RH5 has the ability to induce changes in the RBC cytoskeleton upon binding. This mechanism we propose facilitates the insertion of AMA1-RON complex into the RBC membrane leading to tight junction formation. This knowledge we believe will help in advancing our understanding as well as provide avenue to explore the mechanisms of merozoites invasion
author2 Peter Rainer Preiser
author_facet Peter Rainer Preiser
Yaw, Aniweh
format Theses and Dissertations
author Yaw, Aniweh
author_sort Yaw, Aniweh
title Functional insight the role of plasmodium falciparum reticulocyte blinding like protein homologues : RH2B and RH5 during merozoites invasion
title_short Functional insight the role of plasmodium falciparum reticulocyte blinding like protein homologues : RH2B and RH5 during merozoites invasion
title_full Functional insight the role of plasmodium falciparum reticulocyte blinding like protein homologues : RH2B and RH5 during merozoites invasion
title_fullStr Functional insight the role of plasmodium falciparum reticulocyte blinding like protein homologues : RH2B and RH5 during merozoites invasion
title_full_unstemmed Functional insight the role of plasmodium falciparum reticulocyte blinding like protein homologues : RH2B and RH5 during merozoites invasion
title_sort functional insight the role of plasmodium falciparum reticulocyte blinding like protein homologues : rh2b and rh5 during merozoites invasion
publishDate 2016
url https://hdl.handle.net/10356/69419
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