The microbiome as a target to reduce EV71 pathogenesis and epidemic potential
EV71 is an enterovirus that infects the host through the gut. The gut also houses a diverse array of commensal bacteria. However, not much is understood about viral and bacterial interactions in pathogenesis. We characterised the effects of four species of bacteria on EV71 using in vitro cell model...
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sg-ntu-dr.10356-706132023-02-28T17:59:42Z The microbiome as a target to reduce EV71 pathogenesis and epidemic potential Mohamed Haikal Bin Mohamed Ismail October Michael Sessions School of Biological Sciences Duke-NUS Medical School DRNTU::Science EV71 is an enterovirus that infects the host through the gut. The gut also houses a diverse array of commensal bacteria. However, not much is understood about viral and bacterial interactions in pathogenesis. We characterised the effects of four species of bacteria on EV71 using in vitro cell model assays. The bacteria chosen were either known to have an effect on enteroviruses or form a major constituent of gut microbiome. We observed that exposure to bacteria generally increased viral replication, with Bacillus cereus significantly increasing replication in muscle cells. We also observed that Clostridium difficile significantly increased replication in epithelial cells. Experiments conducted on TLR stimulation and viral replication revealed that intracellular TLR stimulation tend to increase viral expression significantly. The results suggest that EV71 exploits cellular pathways that respond to bacterial stimulation in order to proliferate rapidly. Bachelor of Science in Biological Sciences 2017-05-05T03:56:49Z 2017-05-05T03:56:49Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/70613 en Nanyang Technological University 37 p. application/pdf |
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DRNTU::Science Mohamed Haikal Bin Mohamed Ismail The microbiome as a target to reduce EV71 pathogenesis and epidemic potential |
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EV71 is an enterovirus that infects the host through the gut. The gut also houses a diverse array of commensal bacteria. However, not much is understood about viral and bacterial interactions in pathogenesis. We characterised the effects of four species of bacteria on EV71 using in vitro cell model assays. The bacteria chosen were either known to have an effect on enteroviruses or form a major constituent of gut microbiome. We observed that exposure to bacteria generally increased viral replication, with Bacillus cereus significantly increasing replication in muscle cells. We also observed that Clostridium difficile significantly increased replication in epithelial cells. Experiments conducted on TLR stimulation and viral replication revealed that intracellular TLR stimulation tend to increase viral expression significantly. The results suggest that EV71 exploits cellular pathways that respond to bacterial stimulation in order to proliferate rapidly. |
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October Michael Sessions |
author_facet |
October Michael Sessions Mohamed Haikal Bin Mohamed Ismail |
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Final Year Project |
author |
Mohamed Haikal Bin Mohamed Ismail |
author_sort |
Mohamed Haikal Bin Mohamed Ismail |
title |
The microbiome as a target to reduce EV71 pathogenesis and epidemic potential |
title_short |
The microbiome as a target to reduce EV71 pathogenesis and epidemic potential |
title_full |
The microbiome as a target to reduce EV71 pathogenesis and epidemic potential |
title_fullStr |
The microbiome as a target to reduce EV71 pathogenesis and epidemic potential |
title_full_unstemmed |
The microbiome as a target to reduce EV71 pathogenesis and epidemic potential |
title_sort |
microbiome as a target to reduce ev71 pathogenesis and epidemic potential |
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2017 |
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http://hdl.handle.net/10356/70613 |
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1759856517927927808 |