The microbiome as a target to reduce EV71 pathogenesis and epidemic potential

EV71 is an enterovirus that infects the host through the gut. The gut also houses a diverse array of commensal bacteria. However, not much is understood about viral and bacterial interactions in pathogenesis. We characterised the effects of four species of bacteria on EV71 using in vitro cell model...

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Main Author: Mohamed Haikal Bin Mohamed Ismail
Other Authors: October Michael Sessions
Format: Final Year Project
Language:English
Published: 2017
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Online Access:http://hdl.handle.net/10356/70613
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-706132023-02-28T17:59:42Z The microbiome as a target to reduce EV71 pathogenesis and epidemic potential Mohamed Haikal Bin Mohamed Ismail October Michael Sessions School of Biological Sciences Duke-NUS Medical School DRNTU::Science EV71 is an enterovirus that infects the host through the gut. The gut also houses a diverse array of commensal bacteria. However, not much is understood about viral and bacterial interactions in pathogenesis. We characterised the effects of four species of bacteria on EV71 using in vitro cell model assays. The bacteria chosen were either known to have an effect on enteroviruses or form a major constituent of gut microbiome. We observed that exposure to bacteria generally increased viral replication, with Bacillus cereus significantly increasing replication in muscle cells. We also observed that Clostridium difficile significantly increased replication in epithelial cells. Experiments conducted on TLR stimulation and viral replication revealed that intracellular TLR stimulation tend to increase viral expression significantly. The results suggest that EV71 exploits cellular pathways that respond to bacterial stimulation in order to proliferate rapidly. Bachelor of Science in Biological Sciences 2017-05-05T03:56:49Z 2017-05-05T03:56:49Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/70613 en Nanyang Technological University 37 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science
spellingShingle DRNTU::Science
Mohamed Haikal Bin Mohamed Ismail
The microbiome as a target to reduce EV71 pathogenesis and epidemic potential
description EV71 is an enterovirus that infects the host through the gut. The gut also houses a diverse array of commensal bacteria. However, not much is understood about viral and bacterial interactions in pathogenesis. We characterised the effects of four species of bacteria on EV71 using in vitro cell model assays. The bacteria chosen were either known to have an effect on enteroviruses or form a major constituent of gut microbiome. We observed that exposure to bacteria generally increased viral replication, with Bacillus cereus significantly increasing replication in muscle cells. We also observed that Clostridium difficile significantly increased replication in epithelial cells. Experiments conducted on TLR stimulation and viral replication revealed that intracellular TLR stimulation tend to increase viral expression significantly. The results suggest that EV71 exploits cellular pathways that respond to bacterial stimulation in order to proliferate rapidly.
author2 October Michael Sessions
author_facet October Michael Sessions
Mohamed Haikal Bin Mohamed Ismail
format Final Year Project
author Mohamed Haikal Bin Mohamed Ismail
author_sort Mohamed Haikal Bin Mohamed Ismail
title The microbiome as a target to reduce EV71 pathogenesis and epidemic potential
title_short The microbiome as a target to reduce EV71 pathogenesis and epidemic potential
title_full The microbiome as a target to reduce EV71 pathogenesis and epidemic potential
title_fullStr The microbiome as a target to reduce EV71 pathogenesis and epidemic potential
title_full_unstemmed The microbiome as a target to reduce EV71 pathogenesis and epidemic potential
title_sort microbiome as a target to reduce ev71 pathogenesis and epidemic potential
publishDate 2017
url http://hdl.handle.net/10356/70613
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