Mimicking nanofibrous hybrid substitute for bone tissue engineering
Bone grafts are increasingly used for correcting bone fractures defects. Massive implants usually result in slow healing. The aim of this study is to fabricate nanofibrous scaffolds using Polycaprolactone/silk fibroin/Zinc coated silver nanoparticles (PCL/SF/Ag(Zn)) for bone tissue engineering. Hu...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/72624 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Bone grafts are increasingly used for correcting bone fractures defects. Massive implants usually result
in slow healing. The aim of this study is to fabricate nanofibrous scaffolds using Polycaprolactone/silk
fibroin/Zinc coated silver nanoparticles (PCL/SF/Ag(Zn)) for bone tissue engineering. Human
Mesenchymal Stem cells (hMSCs) were seeded onto the composite scaffolds and allowed to grow for 5,
10 and 15 days. They were then tested for cell proliferation via MTS assay, mineralization qualitatively
via Alizarin red-S dye and live cell morphology via CMFDA dye exclusion methods. The results were that
we found cell proliferation increased with addition of Silk fibroin and even more with addition of Zinc
coated silver nanoparticles. Cell morphology was normal and hence suitable with PCL, but morphology
was more of a more differentiated form with zinc coated silver nanoparticles and silk fibroin samples.
Also, Mineralization was seen to be increased in the samples with silk fibroin, zinc coated silver
nanoparticles. Hence, this suggested that fabricated PCL/SF/Ag (Zn) nanofibrous scaffolds have potential
for use in bone tissue engineering for differentiation of MSCs. |
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