Mimicking nanofibrous hybrid substitute for bone tissue engineering

Bone grafts are increasingly used for correcting bone fractures defects. Massive implants usually result in slow healing. The aim of this study is to fabricate nanofibrous scaffolds using Polycaprolactone/silk fibroin/Zinc coated silver nanoparticles (PCL/SF/Ag(Zn)) for bone tissue engineering. Hu...

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Main Author: Wong, Gabriel Liang Jie
Other Authors: Dinesh Srinivasan
Format: Final Year Project
Language:English
Published: 2017
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Online Access:http://hdl.handle.net/10356/72624
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-726242020-11-01T05:32:42Z Mimicking nanofibrous hybrid substitute for bone tissue engineering Wong, Gabriel Liang Jie Dinesh Srinivasan Lee Kong Chian School of Medicine (LKCMedicine) DRNTU::Science::Medicine Bone grafts are increasingly used for correcting bone fractures defects. Massive implants usually result in slow healing. The aim of this study is to fabricate nanofibrous scaffolds using Polycaprolactone/silk fibroin/Zinc coated silver nanoparticles (PCL/SF/Ag(Zn)) for bone tissue engineering. Human Mesenchymal Stem cells (hMSCs) were seeded onto the composite scaffolds and allowed to grow for 5, 10 and 15 days. They were then tested for cell proliferation via MTS assay, mineralization qualitatively via Alizarin red-S dye and live cell morphology via CMFDA dye exclusion methods. The results were that we found cell proliferation increased with addition of Silk fibroin and even more with addition of Zinc coated silver nanoparticles. Cell morphology was normal and hence suitable with PCL, but morphology was more of a more differentiated form with zinc coated silver nanoparticles and silk fibroin samples. Also, Mineralization was seen to be increased in the samples with silk fibroin, zinc coated silver nanoparticles. Hence, this suggested that fabricated PCL/SF/Ag (Zn) nanofibrous scaffolds have potential for use in bone tissue engineering for differentiation of MSCs. Bachelor of Medicine and Bachelor of Surgery 2017-08-30T08:33:49Z 2017-08-30T08:33:49Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/72624 en 9 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Medicine
spellingShingle DRNTU::Science::Medicine
Wong, Gabriel Liang Jie
Mimicking nanofibrous hybrid substitute for bone tissue engineering
description Bone grafts are increasingly used for correcting bone fractures defects. Massive implants usually result in slow healing. The aim of this study is to fabricate nanofibrous scaffolds using Polycaprolactone/silk fibroin/Zinc coated silver nanoparticles (PCL/SF/Ag(Zn)) for bone tissue engineering. Human Mesenchymal Stem cells (hMSCs) were seeded onto the composite scaffolds and allowed to grow for 5, 10 and 15 days. They were then tested for cell proliferation via MTS assay, mineralization qualitatively via Alizarin red-S dye and live cell morphology via CMFDA dye exclusion methods. The results were that we found cell proliferation increased with addition of Silk fibroin and even more with addition of Zinc coated silver nanoparticles. Cell morphology was normal and hence suitable with PCL, but morphology was more of a more differentiated form with zinc coated silver nanoparticles and silk fibroin samples. Also, Mineralization was seen to be increased in the samples with silk fibroin, zinc coated silver nanoparticles. Hence, this suggested that fabricated PCL/SF/Ag (Zn) nanofibrous scaffolds have potential for use in bone tissue engineering for differentiation of MSCs.
author2 Dinesh Srinivasan
author_facet Dinesh Srinivasan
Wong, Gabriel Liang Jie
format Final Year Project
author Wong, Gabriel Liang Jie
author_sort Wong, Gabriel Liang Jie
title Mimicking nanofibrous hybrid substitute for bone tissue engineering
title_short Mimicking nanofibrous hybrid substitute for bone tissue engineering
title_full Mimicking nanofibrous hybrid substitute for bone tissue engineering
title_fullStr Mimicking nanofibrous hybrid substitute for bone tissue engineering
title_full_unstemmed Mimicking nanofibrous hybrid substitute for bone tissue engineering
title_sort mimicking nanofibrous hybrid substitute for bone tissue engineering
publishDate 2017
url http://hdl.handle.net/10356/72624
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