Identification of pseudomonas aeruginosa via fast PCR in less than 5min
Pseudomonas aeruginosa(PSA) plays a prominent role in infections, especially in chronic illnesses like Chronic Obstructive Pulmonary Disease and the immunocompromised. Rapid detection of PSA would play an important role in reducing mortality in such patients. Using in-house samples of PSA isolate...
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| Other Authors: | |
| Format: | Final Year Project |
| Language: | English |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/72635 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Pseudomonas aeruginosa(PSA) plays a prominent role in infections, especially in chronic illnesses like
Chronic Obstructive Pulmonary Disease and the immunocompromised. Rapid detection of PSA
would play an important role in reducing mortality in such patients. Using in-house samples of PSA
isolated from oral mucosa flora of a healthy young individual, we have managed to detect PSA with
fast PCR, bringing down the time needed for PSA identification from at least an hour to less than
5mins. This is done with good specificity and sensitivity, paving the way for future applications in
rapid bedside testing for the opportunistic pathogen. |
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