Identification of pseudomonas aeruginosa via fast PCR in less than 5min
Pseudomonas aeruginosa(PSA) plays a prominent role in infections, especially in chronic illnesses like Chronic Obstructive Pulmonary Disease and the immunocompromised. Rapid detection of PSA would play an important role in reducing mortality in such patients. Using in-house samples of PSA isolate...
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sg-ntu-dr.10356-726352020-11-01T05:33:06Z Identification of pseudomonas aeruginosa via fast PCR in less than 5min Lee, Soon Hong Yap Peng Huat Eric Lee Kong Chian School of Medicine (LKCMedicine) DRNTU::Science::Medicine Pseudomonas aeruginosa(PSA) plays a prominent role in infections, especially in chronic illnesses like Chronic Obstructive Pulmonary Disease and the immunocompromised. Rapid detection of PSA would play an important role in reducing mortality in such patients. Using in-house samples of PSA isolated from oral mucosa flora of a healthy young individual, we have managed to detect PSA with fast PCR, bringing down the time needed for PSA identification from at least an hour to less than 5mins. This is done with good specificity and sensitivity, paving the way for future applications in rapid bedside testing for the opportunistic pathogen. Bachelor of Medicine and Bachelor of Surgery 2017-08-31T04:04:53Z 2017-08-31T04:04:53Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/72635 en 17 p. application/pdf |
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Asia |
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DRNTU::Science::Medicine Lee, Soon Hong Identification of pseudomonas aeruginosa via fast PCR in less than 5min |
| description |
Pseudomonas aeruginosa(PSA) plays a prominent role in infections, especially in chronic illnesses like
Chronic Obstructive Pulmonary Disease and the immunocompromised. Rapid detection of PSA
would play an important role in reducing mortality in such patients. Using in-house samples of PSA
isolated from oral mucosa flora of a healthy young individual, we have managed to detect PSA with
fast PCR, bringing down the time needed for PSA identification from at least an hour to less than
5mins. This is done with good specificity and sensitivity, paving the way for future applications in
rapid bedside testing for the opportunistic pathogen. |
| author2 |
Yap Peng Huat Eric |
| author_facet |
Yap Peng Huat Eric Lee, Soon Hong |
| format |
Final Year Project |
| author |
Lee, Soon Hong |
| author_sort |
Lee, Soon Hong |
| title |
Identification of pseudomonas aeruginosa via fast PCR in less than 5min |
| title_short |
Identification of pseudomonas aeruginosa via fast PCR in less than 5min |
| title_full |
Identification of pseudomonas aeruginosa via fast PCR in less than 5min |
| title_fullStr |
Identification of pseudomonas aeruginosa via fast PCR in less than 5min |
| title_full_unstemmed |
Identification of pseudomonas aeruginosa via fast PCR in less than 5min |
| title_sort |
identification of pseudomonas aeruginosa via fast pcr in less than 5min |
| publishDate |
2017 |
| url |
http://hdl.handle.net/10356/72635 |
| _version_ |
1683493633459748864 |