Cryo-EM structure of the chloroplast ribosome

Ribosomes are the universal machines which perform mRNA translation inside the cell. Chloroplasts harbor bacterial-like 70S ribosome but have acquired five plastid-specific ribosomal proteins (PSRPs) namely cL37, cL38, cS22, cS23 and bTHXc, extensions in the plastid ribosomal proteins (PRPs) and rRN...

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Main Author: Tofayel Ahmed
Other Authors: Shashi Bhushan
Format: Theses and Dissertations
Language:English
Published: 2018
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Online Access:http://hdl.handle.net/10356/75853
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-758532023-02-28T18:35:17Z Cryo-EM structure of the chloroplast ribosome Tofayel Ahmed Shashi Bhushan School of Biological Sciences DRNTU::Science::Biological sciences Ribosomes are the universal machines which perform mRNA translation inside the cell. Chloroplasts harbor bacterial-like 70S ribosome but have acquired five plastid-specific ribosomal proteins (PSRPs) namely cL37, cL38, cS22, cS23 and bTHXc, extensions in the plastid ribosomal proteins (PRPs) and rRNA expansions and deletions. Here, a complete structure of the 70S spinach chloroplast ribosome (cp-ribosome) has been determined using cryo-electron microscopy and single particle analysis. The structures of the large (LSU) and small subunits (SSU) are resolved to 3.3 and 3.7 Å, respectively, demonstrating that the overall architecture of cp-ribosome is similar to the bacterial counterpart. The localization and modeling of the PSRPs, PRP-extensions and remodeled rRNAs suggest about the probable functions of these altered elements in the contexts of cp-ribosome structure and translation regulation inside chloroplasts. The mode of interaction of the hibernation factor, plastid pY, which is present at the subunit interface is also discussed. ​Doctor of Philosophy (SBS) 2018-06-20T03:53:29Z 2018-06-20T03:53:29Z 2018 Thesis Tofayel Ahmed. (2018). Cryo-EM structure of the chloroplast ribosome. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/75853 10.32657/10356/75853 en 153 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Tofayel Ahmed
Cryo-EM structure of the chloroplast ribosome
description Ribosomes are the universal machines which perform mRNA translation inside the cell. Chloroplasts harbor bacterial-like 70S ribosome but have acquired five plastid-specific ribosomal proteins (PSRPs) namely cL37, cL38, cS22, cS23 and bTHXc, extensions in the plastid ribosomal proteins (PRPs) and rRNA expansions and deletions. Here, a complete structure of the 70S spinach chloroplast ribosome (cp-ribosome) has been determined using cryo-electron microscopy and single particle analysis. The structures of the large (LSU) and small subunits (SSU) are resolved to 3.3 and 3.7 Å, respectively, demonstrating that the overall architecture of cp-ribosome is similar to the bacterial counterpart. The localization and modeling of the PSRPs, PRP-extensions and remodeled rRNAs suggest about the probable functions of these altered elements in the contexts of cp-ribosome structure and translation regulation inside chloroplasts. The mode of interaction of the hibernation factor, plastid pY, which is present at the subunit interface is also discussed.
author2 Shashi Bhushan
author_facet Shashi Bhushan
Tofayel Ahmed
format Theses and Dissertations
author Tofayel Ahmed
author_sort Tofayel Ahmed
title Cryo-EM structure of the chloroplast ribosome
title_short Cryo-EM structure of the chloroplast ribosome
title_full Cryo-EM structure of the chloroplast ribosome
title_fullStr Cryo-EM structure of the chloroplast ribosome
title_full_unstemmed Cryo-EM structure of the chloroplast ribosome
title_sort cryo-em structure of the chloroplast ribosome
publishDate 2018
url http://hdl.handle.net/10356/75853
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