Effect of micro-RNA 150 and 885 on liver fibrosis

• Main Author: Ali, Ashik
• Other Authors: Dang Thuy Tram
• Format: Final Year Project
• Language: English
• Published: 2019
• Subjects: DRNTU::Science::Biological sciences; DRNTU::Engineering::Bioengineering
• Online Access: http://hdl.handle.net/10356/78035
• Institution: Nanyang Technological University

This paper analyzes the effects of MiRNA 150 and 885 on liver fibrosis. To establish if these MiRNAs are pro-fibrotic or anti-fibrotic, fibrotic expression of HSC Activation and Quiescence Markers are quantified after over-expressing the MiRNAs in LX2 cells. Our results suggest that MiRNA 150 is pro-fibrotic due to the relative over-expression of classical HSC activation markers while MiRNA 885 tends to be inconclusive with inconsistent results. This paper also serves as a guide for cloning and testing MiRNAs in cells for any gene expression in-vitro. MiRNA 150 can be further studied on a protein level for potential therapeutic value to those suffering from Liver fibrosis.