Biomolecule nanosensing

Detection of genetically modified food through Real-Time Polymerase Chain Reaction (RT PCR) involves the use of expensive fluorescence labels. In this study, comparison between three nano-graphene oxide (GO) materials (HO Nano-400, TO SGNF, and HO PyroG) was conducted in order to find out which nano...

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Main Author: Neo, Yong Yee
Other Authors: Alessandra Bonanni
Format: Final Year Project
Language:English
Published: 2019
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Online Access:http://hdl.handle.net/10356/78959
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-789592023-02-28T23:14:25Z Biomolecule nanosensing Neo, Yong Yee Alessandra Bonanni School of Physical and Mathematical Sciences Science::Chemistry Detection of genetically modified food through Real-Time Polymerase Chain Reaction (RT PCR) involves the use of expensive fluorescence labels. In this study, comparison between three nano-graphene oxide (GO) materials (HO Nano-400, TO SGNF, and HO PyroG) was conducted in order to find out which nano-GO material would be a more suitable candidate as electrochemical labels in place of the traditional fluorescence labels. Electrochemical characterization including X-ray photoelectron spectroscopy (XPS), cyclic voltammetry, differential pulse voltammetry, square-wave voltammetry as well as electrochemical impedance spectroscopy (EIS) was performed across varying concentrations of nano-GO. Conjugation of nano-GO materials with single-stranded DNA via covalent bonding was carried out and electrochemical characterization was repeated on the conjugated nano-GO materials. A significant electrochemical signal for all nano-GO materials was still observed after conjugation with single-stranded DNA corresponding to the DNA primer sequence. Hybridization of one of the conjugated nano-GOs (HO PyroG) with complementary strand (Sequence 1) was carried out as a preliminary study due to its better electroanalytical performance. Electrochemical characterization was also performed where a reduction of electrochemical signal of the hybrid was observed. Further characterization was performed using techniques including attenuated total reflectance fourier transform infrared (ATR-FTIR) spectroscopy and fluorescence were carried out on these materials to monitor if the conjugation of sequence to nano-GO materials was successful. With that, the findings in this study are essential toward finding a strategy for electrochemical PCR, in place of the traditional and thus expensive RT PCR. Bachelor of Science in Chemistry and Biological Chemistry 2019-11-13T00:55:40Z 2019-11-13T00:55:40Z 2019 Final Year Project (FYP) http://hdl.handle.net/10356/78959 en 41 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Chemistry
spellingShingle Science::Chemistry
Neo, Yong Yee
Biomolecule nanosensing
description Detection of genetically modified food through Real-Time Polymerase Chain Reaction (RT PCR) involves the use of expensive fluorescence labels. In this study, comparison between three nano-graphene oxide (GO) materials (HO Nano-400, TO SGNF, and HO PyroG) was conducted in order to find out which nano-GO material would be a more suitable candidate as electrochemical labels in place of the traditional fluorescence labels. Electrochemical characterization including X-ray photoelectron spectroscopy (XPS), cyclic voltammetry, differential pulse voltammetry, square-wave voltammetry as well as electrochemical impedance spectroscopy (EIS) was performed across varying concentrations of nano-GO. Conjugation of nano-GO materials with single-stranded DNA via covalent bonding was carried out and electrochemical characterization was repeated on the conjugated nano-GO materials. A significant electrochemical signal for all nano-GO materials was still observed after conjugation with single-stranded DNA corresponding to the DNA primer sequence. Hybridization of one of the conjugated nano-GOs (HO PyroG) with complementary strand (Sequence 1) was carried out as a preliminary study due to its better electroanalytical performance. Electrochemical characterization was also performed where a reduction of electrochemical signal of the hybrid was observed. Further characterization was performed using techniques including attenuated total reflectance fourier transform infrared (ATR-FTIR) spectroscopy and fluorescence were carried out on these materials to monitor if the conjugation of sequence to nano-GO materials was successful. With that, the findings in this study are essential toward finding a strategy for electrochemical PCR, in place of the traditional and thus expensive RT PCR.
author2 Alessandra Bonanni
author_facet Alessandra Bonanni
Neo, Yong Yee
format Final Year Project
author Neo, Yong Yee
author_sort Neo, Yong Yee
title Biomolecule nanosensing
title_short Biomolecule nanosensing
title_full Biomolecule nanosensing
title_fullStr Biomolecule nanosensing
title_full_unstemmed Biomolecule nanosensing
title_sort biomolecule nanosensing
publishDate 2019
url http://hdl.handle.net/10356/78959
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