Nitric oxide treatment for the control of reverse osmosis membrane biofouling

Biofouling remains a key challenge for membrane-based water treatment systems. This study investigated the dispersal potential of the nitric oxide (NO) donor compound, PROLI NONOate, on single- and mixed-species biofilms formed by bacteria isolated from industrial membrane bioreactor and reverse osm...

Full description

Saved in:
Bibliographic Details
Main Authors: Fane, Anthony G., Kjelleberg, Staffan, Rice, Scott A., Barnes, Robert J., Low, Jiun Hui, Bandi, Ratnaharika R., Tay, Martin, Chua, Felicia, Aung, Theingi
Other Authors: Nojiri, H.
Format: Article
Language:English
Published: 2015
Subjects:
Online Access:https://hdl.handle.net/10356/79458
http://hdl.handle.net/10220/26194
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-79458
record_format dspace
spelling sg-ntu-dr.10356-794582022-02-16T16:29:17Z Nitric oxide treatment for the control of reverse osmosis membrane biofouling Fane, Anthony G. Kjelleberg, Staffan Rice, Scott A. Barnes, Robert J. Low, Jiun Hui Bandi, Ratnaharika R. Tay, Martin Chua, Felicia Aung, Theingi Nojiri, H. School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering Advanced Environmental Biotechnology Centre (AEBC) Nanyang Environment and Water Research Institute Singapore Membrane Technology Centre DRNTU::Science::Biological sciences::Microbiology Biofouling remains a key challenge for membrane-based water treatment systems. This study investigated the dispersal potential of the nitric oxide (NO) donor compound, PROLI NONOate, on single- and mixed-species biofilms formed by bacteria isolated from industrial membrane bioreactor and reverse osmosis (RO) membranes. The potential of PROLI NONOate to control RO membrane biofouling was also examined. Confocal microscopy revealed that PROLI NONOate exposure induced biofilm dispersal in all but two of the bacteria tested and successfully dispersed mixed-species biofilms. The addition of 40 μM PROLI NONOate at 24-h intervals to a laboratory-scale RO system led to a 92% reduction in the rate of biofouling (pressure rise over a given period) by a bacterial community cultured from an industrial RO membrane. Confocal microscopy and extracellular polymeric substances (EPS) extraction revealed that PROLI NONOate treatment led to a 48% reduction in polysaccharides, a 66% reduction in proteins, and a 29% reduction in microbial cells compared to the untreated control. A reduction in biofilm surface coverage (59% compared to 98%, treated compared to control) and average thickness (20 μm compared to 26 μm, treated compared to control) was also observed. The addition of PROLI NONOate led to a 22% increase in the time required for the RO module to reach its maximum transmembrane pressure (TMP), further indicating that NO treatment delayed fouling. Pyrosequencing analysis revealed that the NO treatment did not significantly alter the microbial community composition of the membrane biofilm. These results present strong evidence for the application of PROLI NONOate for prevention of RO biofouling. Published version 2015-07-01T08:54:18Z 2019-12-06T13:25:50Z 2015-07-01T08:54:18Z 2019-12-06T13:25:50Z 2015 2015 Journal Article Barnes, R. J., Low, J. H., Bandi, R. R., Tay, M., Chua, F., Aung, T., et al. (2015). Nitric oxide treatment for the control of reverse osmosis membrane biofouling. Applied and environmental microbiology, 81(7), 2515-2524. https://hdl.handle.net/10356/79458 http://hdl.handle.net/10220/26194 10.1128/AEM.03404-14 25636842 en Applied and environmental microbiology © 2015 American Society for Microbiology. This paper was published in Applied and Environmental Microbiology and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology. The paper can be found at the following official DOI: [http://dx.doi.org/10.1128/AEM.03404-14]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology
spellingShingle DRNTU::Science::Biological sciences::Microbiology
Fane, Anthony G.
Kjelleberg, Staffan
Rice, Scott A.
Barnes, Robert J.
Low, Jiun Hui
Bandi, Ratnaharika R.
Tay, Martin
Chua, Felicia
Aung, Theingi
Nitric oxide treatment for the control of reverse osmosis membrane biofouling
description Biofouling remains a key challenge for membrane-based water treatment systems. This study investigated the dispersal potential of the nitric oxide (NO) donor compound, PROLI NONOate, on single- and mixed-species biofilms formed by bacteria isolated from industrial membrane bioreactor and reverse osmosis (RO) membranes. The potential of PROLI NONOate to control RO membrane biofouling was also examined. Confocal microscopy revealed that PROLI NONOate exposure induced biofilm dispersal in all but two of the bacteria tested and successfully dispersed mixed-species biofilms. The addition of 40 μM PROLI NONOate at 24-h intervals to a laboratory-scale RO system led to a 92% reduction in the rate of biofouling (pressure rise over a given period) by a bacterial community cultured from an industrial RO membrane. Confocal microscopy and extracellular polymeric substances (EPS) extraction revealed that PROLI NONOate treatment led to a 48% reduction in polysaccharides, a 66% reduction in proteins, and a 29% reduction in microbial cells compared to the untreated control. A reduction in biofilm surface coverage (59% compared to 98%, treated compared to control) and average thickness (20 μm compared to 26 μm, treated compared to control) was also observed. The addition of PROLI NONOate led to a 22% increase in the time required for the RO module to reach its maximum transmembrane pressure (TMP), further indicating that NO treatment delayed fouling. Pyrosequencing analysis revealed that the NO treatment did not significantly alter the microbial community composition of the membrane biofilm. These results present strong evidence for the application of PROLI NONOate for prevention of RO biofouling.
author2 Nojiri, H.
author_facet Nojiri, H.
Fane, Anthony G.
Kjelleberg, Staffan
Rice, Scott A.
Barnes, Robert J.
Low, Jiun Hui
Bandi, Ratnaharika R.
Tay, Martin
Chua, Felicia
Aung, Theingi
format Article
author Fane, Anthony G.
Kjelleberg, Staffan
Rice, Scott A.
Barnes, Robert J.
Low, Jiun Hui
Bandi, Ratnaharika R.
Tay, Martin
Chua, Felicia
Aung, Theingi
author_sort Fane, Anthony G.
title Nitric oxide treatment for the control of reverse osmosis membrane biofouling
title_short Nitric oxide treatment for the control of reverse osmosis membrane biofouling
title_full Nitric oxide treatment for the control of reverse osmosis membrane biofouling
title_fullStr Nitric oxide treatment for the control of reverse osmosis membrane biofouling
title_full_unstemmed Nitric oxide treatment for the control of reverse osmosis membrane biofouling
title_sort nitric oxide treatment for the control of reverse osmosis membrane biofouling
publishDate 2015
url https://hdl.handle.net/10356/79458
http://hdl.handle.net/10220/26194
_version_ 1725985627479998464