Detection of Listeria monocytogenes by using the polymerase chain reaction

A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other spec...

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Main Authors: Rotbart, Harley A., Blaser, Martin J., Ellison III, Richard T., Bessesen, Mary T., Luo, Qian
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2014
Subjects:
Online Access:https://hdl.handle.net/10356/79472
http://hdl.handle.net/10220/18733
http://aem.asm.org/content/56/9/2930.full.pdf+html
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-794722023-12-29T06:46:13Z Detection of Listeria monocytogenes by using the polymerase chain reaction Rotbart, Harley A. Blaser, Martin J. Ellison III, Richard T. Bessesen, Mary T. Luo, Qian School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Chemical processes A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains. Accepted version 2014-01-29T01:54:50Z 2019-12-06T13:26:09Z 2014-01-29T01:54:50Z 2019-12-06T13:26:09Z 1990 1990 Journal Article Bessesen, M. T., Luo, Q., Rotbart, H. A., Blaser, M. J., & Ellison III, R. T. (1990). Detection of Listeria monocytogenes by using the polymerase chain reaction. Applied and environmental microbiology, 56(9), 2930-2932. https://hdl.handle.net/10356/79472 http://hdl.handle.net/10220/18733 http://aem.asm.org/content/56/9/2930.full.pdf+html en Applied and environmental microbiology © 1990 American Society for Microbiology. This is the author created version of a work that has been peer reviewed and accepted for publication by Applied And Environmental Microbiology, American Society for Microbiology. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://aem.asm.org/content/56/9/2930.full.pdf+html]. 3 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Chemical engineering::Chemical processes
spellingShingle DRNTU::Engineering::Chemical engineering::Chemical processes
Rotbart, Harley A.
Blaser, Martin J.
Ellison III, Richard T.
Bessesen, Mary T.
Luo, Qian
Detection of Listeria monocytogenes by using the polymerase chain reaction
description A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Rotbart, Harley A.
Blaser, Martin J.
Ellison III, Richard T.
Bessesen, Mary T.
Luo, Qian
format Article
author Rotbart, Harley A.
Blaser, Martin J.
Ellison III, Richard T.
Bessesen, Mary T.
Luo, Qian
author_sort Rotbart, Harley A.
title Detection of Listeria monocytogenes by using the polymerase chain reaction
title_short Detection of Listeria monocytogenes by using the polymerase chain reaction
title_full Detection of Listeria monocytogenes by using the polymerase chain reaction
title_fullStr Detection of Listeria monocytogenes by using the polymerase chain reaction
title_full_unstemmed Detection of Listeria monocytogenes by using the polymerase chain reaction
title_sort detection of listeria monocytogenes by using the polymerase chain reaction
publishDate 2014
url https://hdl.handle.net/10356/79472
http://hdl.handle.net/10220/18733
http://aem.asm.org/content/56/9/2930.full.pdf+html
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