Detection of Listeria monocytogenes by using the polymerase chain reaction

• Main Authors: Rotbart, Harley A., Blaser, Martin J., Ellison III, Richard T., Bessesen, Mary T., Luo, Qian
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Engineering::Chemical engineering::Chemical processes
• Online Access: https://hdl.handle.net/10356/79472; http://hdl.handle.net/10220/18733; http://aem.asm.org/content/56/9/2930.full.pdf+html
• Institution: Nanyang Technological University

A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other spec...