Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillinresistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on...

Full description

Saved in:
Bibliographic Details
Main Authors: Wang, Zhijuan, Zhang, Juan, Chen, Peng, Zhou, Xiaozhu, Yang, Yanli, Wu, Shixin, Han, Yu, Wang, Lianhui, Boey, Freddy Yin Chiang, Niu, Li, Zhang, Qichun, Bo, Liedberg, Zhang, Hua
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2012
Subjects:
DRNTU::Science::Medicine::Biosensors
Online Access:https://hdl.handle.net/10356/79512
http://hdl.handle.net/10220/8333
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
Description
Summary:Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillinresistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10−13 M, i.e., 100 fM) and selectivity.

Similar Items