Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

• Main Authors: Wang, Zhijuan, Zhang, Juan, Chen, Peng, Zhou, Xiaozhu, Yang, Yanli, Wu, Shixin, Han, Yu, Wang, Lianhui, Boey, Freddy Yin Chiang, Niu, Li, Zhang, Qichun, Bo, Liedberg, Zhang, Hua
• Other Authors: School of Materials Science & Engineering
• Format: Article
• Language: English
• Published: 2012
• Subjects: DRNTU::Science::Medicine::Biosensors
• Online Access: https://hdl.handle.net/10356/79512; http://hdl.handle.net/10220/8333
• Institution: Nanyang Technological University

Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillinresistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10−13 M, i.e., 100 fM) and selectivity.