Detection of evolutionarily distinct Avian Influenza A Viruses in Antarctica

Distinct lineages of avian influenza viruses (AIVs) are harbored by spatially segregated birds, yet significant surveillance gaps exist around the globe. Virtually nothing is known from the Antarctic. Using virus culture, molecular analysis, full genome sequencing, and serology of samples from Adéli...

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Main Authors: Hurt, Aeron C., Vijaykrishna, Dhanasekaran, Butler, Jeffrey, Baas, Chantal, Maurer-Stroh, Sebastian, Silva-de-la-Fuente, M. Carolina, Medina-Vogel, Gonzalo, Olsen, Bjorn, Kelso, Anne, Barr, Ian G., González-Acuña, Daniel
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/79705
http://hdl.handle.net/10220/20318
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-797052023-02-28T16:58:28Z Detection of evolutionarily distinct Avian Influenza A Viruses in Antarctica Hurt, Aeron C. Vijaykrishna, Dhanasekaran Butler, Jeffrey Baas, Chantal Maurer-Stroh, Sebastian Silva-de-la-Fuente, M. Carolina Medina-Vogel, Gonzalo Olsen, Bjorn Kelso, Anne Barr, Ian G. González-Acuña, Daniel School of Biological Sciences DRNTU::Science::Biological sciences Distinct lineages of avian influenza viruses (AIVs) are harbored by spatially segregated birds, yet significant surveillance gaps exist around the globe. Virtually nothing is known from the Antarctic. Using virus culture, molecular analysis, full genome sequencing, and serology of samples from Adélie penguins in Antarctica, we confirmed infection by H11N2 subtype AIVs. Their genetic segments were distinct from all known contemporary influenza viruses, including South American AIVs, suggesting spatial separation from other lineages. Only in the matrix and polymerase acidic gene phylogenies did the Antarctic sequences form a sister relationship to South American AIVs, whereas distant phylogenetic relationships were evident in all other gene segments. Interestingly, their neuraminidase genes formed a distant relationship to all avian and human influenza lineages, and the polymerase basic 1 and polymerase acidic formed a sister relationship to the equine H3N8 influenza virus lineage that emerged during 1963 and whose avian origins were previously unknown. We also estimated that each gene segment had diverged for 49 to 80 years from its most closely related sequences, highlighting a significant gap in our AIV knowledge in the region. We also show that the receptor binding properties of the H11N2 viruses are predominantly avian and that they were unable to replicate efficiently in experimentally inoculated ferrets, suggesting their continuous evolution in avian hosts. These findings add substantially to our understanding of both the ecology and the intra- and intercontinental movement of Antarctic AIVs and highlight the potential risk of an incursion of highly pathogenic AIVs into this fragile environment. ASTAR (Agency for Sci., Tech. and Research, S’pore) Published version 2014-08-18T02:38:13Z 2019-12-06T13:31:24Z 2014-08-18T02:38:13Z 2019-12-06T13:31:24Z 2014 2014 Journal Article Hurt, A. C., Vijaykrishna, D., Butler, J., Baas, C., Maurer-Stroh, S., Silva-de-la-Fuente, M. C., et al. (2014). Detection of Evolutionarily Distinct Avian Influenza A Viruses in Antarctica. mBio, 5(3), e01098-14-. 2150-7511 https://hdl.handle.net/10356/79705 http://hdl.handle.net/10220/20318 10.1128/mBio.01098-14 24803521 en mBio Copyright © 2014 Hurt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Hurt, Aeron C.
Vijaykrishna, Dhanasekaran
Butler, Jeffrey
Baas, Chantal
Maurer-Stroh, Sebastian
Silva-de-la-Fuente, M. Carolina
Medina-Vogel, Gonzalo
Olsen, Bjorn
Kelso, Anne
Barr, Ian G.
González-Acuña, Daniel
Detection of evolutionarily distinct Avian Influenza A Viruses in Antarctica
description Distinct lineages of avian influenza viruses (AIVs) are harbored by spatially segregated birds, yet significant surveillance gaps exist around the globe. Virtually nothing is known from the Antarctic. Using virus culture, molecular analysis, full genome sequencing, and serology of samples from Adélie penguins in Antarctica, we confirmed infection by H11N2 subtype AIVs. Their genetic segments were distinct from all known contemporary influenza viruses, including South American AIVs, suggesting spatial separation from other lineages. Only in the matrix and polymerase acidic gene phylogenies did the Antarctic sequences form a sister relationship to South American AIVs, whereas distant phylogenetic relationships were evident in all other gene segments. Interestingly, their neuraminidase genes formed a distant relationship to all avian and human influenza lineages, and the polymerase basic 1 and polymerase acidic formed a sister relationship to the equine H3N8 influenza virus lineage that emerged during 1963 and whose avian origins were previously unknown. We also estimated that each gene segment had diverged for 49 to 80 years from its most closely related sequences, highlighting a significant gap in our AIV knowledge in the region. We also show that the receptor binding properties of the H11N2 viruses are predominantly avian and that they were unable to replicate efficiently in experimentally inoculated ferrets, suggesting their continuous evolution in avian hosts. These findings add substantially to our understanding of both the ecology and the intra- and intercontinental movement of Antarctic AIVs and highlight the potential risk of an incursion of highly pathogenic AIVs into this fragile environment.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Hurt, Aeron C.
Vijaykrishna, Dhanasekaran
Butler, Jeffrey
Baas, Chantal
Maurer-Stroh, Sebastian
Silva-de-la-Fuente, M. Carolina
Medina-Vogel, Gonzalo
Olsen, Bjorn
Kelso, Anne
Barr, Ian G.
González-Acuña, Daniel
format Article
author Hurt, Aeron C.
Vijaykrishna, Dhanasekaran
Butler, Jeffrey
Baas, Chantal
Maurer-Stroh, Sebastian
Silva-de-la-Fuente, M. Carolina
Medina-Vogel, Gonzalo
Olsen, Bjorn
Kelso, Anne
Barr, Ian G.
González-Acuña, Daniel
author_sort Hurt, Aeron C.
title Detection of evolutionarily distinct Avian Influenza A Viruses in Antarctica
title_short Detection of evolutionarily distinct Avian Influenza A Viruses in Antarctica
title_full Detection of evolutionarily distinct Avian Influenza A Viruses in Antarctica
title_fullStr Detection of evolutionarily distinct Avian Influenza A Viruses in Antarctica
title_full_unstemmed Detection of evolutionarily distinct Avian Influenza A Viruses in Antarctica
title_sort detection of evolutionarily distinct avian influenza a viruses in antarctica
publishDate 2014
url https://hdl.handle.net/10356/79705
http://hdl.handle.net/10220/20318
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