A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease

• Main Authors: Ivanov, Yury V., Shariat, Nikki, Register, Karen B., Linz, Bodo, Rivera, Israel, Hu, Kai, Dudley, Edward G., Harvill, Eric Thomas
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2015
• Subjects: Bordetella pseudohinzii; Bacteria; Genome editing; Protospacer; Type II CRISPR; Cas9; SpyCas9; GC-content; HGT
• Online Access: https://hdl.handle.net/10356/80936; http://hdl.handle.net/10220/38980
• Institution: Nanyang Technological University

Background: Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria. These systems provide adaptive immunity against mobile genetic elements specified by the spacer sequences stored within the CRISPR. Methods: The CRI...