Functional VEGFA knockdown with artificial 3′-tailed mirtrons defined by 5′ splice site and branch point

• Main Authors: Kock, Kian Hong, Kong, Kiat Whye, Hoon, Shawn, Seow, Yiqi
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: Biological Sciences
• Online Access: https://hdl.handle.net/10356/81115; http://hdl.handle.net/10220/39104
• Institution: Nanyang Technological University

Mirtrons are introns that form pre-miRNA hairpins after splicing to produce RNA interference (RNAi) effectors distinct from Drosha-dependent intronic miRNAs, and will be especially useful for co-delivery of coding genes and RNAi. A specific family of mirtrons – 3′-tailed mirtrons – has hairpins prec...