Functional VEGFA knockdown with artificial 3′-tailed mirtrons defined by 5′ splice site and branch point

• المؤلفون الرئيسيون: Kock, Kian Hong, Kong, Kiat Whye, Hoon, Shawn, Seow, Yiqi
• مؤلفون آخرون: School of Biological Sciences
• التنسيق: مقال
• اللغة: English
• منشور في: 2015
• الموضوعات: Biological Sciences
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/81115; http://hdl.handle.net/10220/39104
• المؤسسة: Nanyang Technological University

Mirtrons are introns that form pre-miRNA hairpins after splicing to produce RNA interference (RNAi) effectors distinct from Drosha-dependent intronic miRNAs, and will be especially useful for co-delivery of coding genes and RNAi. A specific family of mirtrons – 3′-tailed mirtrons – has hairpins prec...