Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments

Background: Previous studies of higher order chromatin organization in nuclei of mammalian species revealed both structural consistency and species-specific differences between cell lines and during early embryonic development. Here, we extended our studies to nuclear landscapes in the human myelop...

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Main Authors: Markaki, Yolanda, Ferrari, Sergio, Cremer, Marion, Hübner, Barbara, Lomiento, Mariana, Mammoli, Fabiana, Illner, Doris, Cremer, Thomas
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2015
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Online Access:https://hdl.handle.net/10356/81119
http://hdl.handle.net/10220/39099
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-811192023-02-28T16:58:41Z Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments Markaki, Yolanda Ferrari, Sergio Cremer, Marion Hübner, Barbara Lomiento, Mariana Mammoli, Fabiana Illner, Doris Cremer, Thomas School of Biological Sciences Myelopoiesis Somatic cell differentiation Nuclear architecture Active nuclear compartment Interchromatin compartment Perichromatin region Super-resolution microscopy Electron microscopy Chromatin domain Chromatin density classification Background: Previous studies of higher order chromatin organization in nuclei of mammalian species revealed both structural consistency and species-specific differences between cell lines and during early embryonic development. Here, we extended our studies to nuclear landscapes in the human myelopoietic lineage representing a somatic cell differentiation system. Our longterm goal is a search for structural features of nuclei, which are restricted to certain cell types/species, as compared to features, which are evolutionary highly conserved, arguing for their basic functional roles in nuclear organization. Results: Common human hematopoietic progenitors, myeloid precursor cells, differentiated monocytes and granulocytes analyzed by super-resolution fluorescence microscopy and electron microscopy revealed profound differences with respect to global chromatin arrangements, the nuclear space occupied by the interchromatin compartment and the distribution of nuclear pores. In contrast, we noted a consistent organization in all cell types with regard to two co-aligned networks, an active (ANC) and an inactive (INC) nuclear compartment delineated by functionally relevant hallmarks. The ANC is enriched in active RNA polymerase II, splicing speckles and histone signatures for transcriptionally competent chromatin (H3K4me3), whereas the INC carries marks for repressed chromatin (H3K9me3). Conclusions: Our findings substantiate the conservation of the recently published ANC-INC network model of mammalian nuclear organization during human myelopoiesis irrespective of profound changes of the global nuclear architecture observed during this differentiation process. According to this model, two spatially co-aligned and functionally interacting active and inactive nuclear compartments (ANC and INC) pervade the nuclear space. Published version 2015-12-16T08:19:08Z 2019-12-06T14:21:49Z 2015-12-16T08:19:08Z 2019-12-06T14:21:49Z 2015 Journal Article Hübner, B., Lomiento, M., Mammoli, F., Illner, D., Markaki, Y., Ferrari, S., et al. (2015). Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments. Epigenetics & Chromatin, 8, 47-. 1756-8935 https://hdl.handle.net/10356/81119 http://hdl.handle.net/10220/39099 10.1186/s13072-015-0038-0 26579212 en Epigenetics & Chromatin © 2015 Hübner et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. 21 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Myelopoiesis
Somatic cell differentiation
Nuclear architecture
Active nuclear compartment
Interchromatin compartment
Perichromatin region
Super-resolution microscopy
Electron microscopy
Chromatin domain
Chromatin density classification
spellingShingle Myelopoiesis
Somatic cell differentiation
Nuclear architecture
Active nuclear compartment
Interchromatin compartment
Perichromatin region
Super-resolution microscopy
Electron microscopy
Chromatin domain
Chromatin density classification
Markaki, Yolanda
Ferrari, Sergio
Cremer, Marion
Hübner, Barbara
Lomiento, Mariana
Mammoli, Fabiana
Illner, Doris
Cremer, Thomas
Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments
description Background: Previous studies of higher order chromatin organization in nuclei of mammalian species revealed both structural consistency and species-specific differences between cell lines and during early embryonic development. Here, we extended our studies to nuclear landscapes in the human myelopoietic lineage representing a somatic cell differentiation system. Our longterm goal is a search for structural features of nuclei, which are restricted to certain cell types/species, as compared to features, which are evolutionary highly conserved, arguing for their basic functional roles in nuclear organization. Results: Common human hematopoietic progenitors, myeloid precursor cells, differentiated monocytes and granulocytes analyzed by super-resolution fluorescence microscopy and electron microscopy revealed profound differences with respect to global chromatin arrangements, the nuclear space occupied by the interchromatin compartment and the distribution of nuclear pores. In contrast, we noted a consistent organization in all cell types with regard to two co-aligned networks, an active (ANC) and an inactive (INC) nuclear compartment delineated by functionally relevant hallmarks. The ANC is enriched in active RNA polymerase II, splicing speckles and histone signatures for transcriptionally competent chromatin (H3K4me3), whereas the INC carries marks for repressed chromatin (H3K9me3). Conclusions: Our findings substantiate the conservation of the recently published ANC-INC network model of mammalian nuclear organization during human myelopoiesis irrespective of profound changes of the global nuclear architecture observed during this differentiation process. According to this model, two spatially co-aligned and functionally interacting active and inactive nuclear compartments (ANC and INC) pervade the nuclear space.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Markaki, Yolanda
Ferrari, Sergio
Cremer, Marion
Hübner, Barbara
Lomiento, Mariana
Mammoli, Fabiana
Illner, Doris
Cremer, Thomas
format Article
author Markaki, Yolanda
Ferrari, Sergio
Cremer, Marion
Hübner, Barbara
Lomiento, Mariana
Mammoli, Fabiana
Illner, Doris
Cremer, Thomas
author_sort Markaki, Yolanda
title Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments
title_short Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments
title_full Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments
title_fullStr Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments
title_full_unstemmed Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments
title_sort remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments
publishDate 2015
url https://hdl.handle.net/10356/81119
http://hdl.handle.net/10220/39099
_version_ 1759856007203258368