Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation

Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation

Nonenzymatic deamidation occurs readily under the condition of trypsin digestion, resulting in the identification of many artificial deamidation sites. To evaluate the effect of trypsin digestion buffers on artificial deamidation, we compared the three commonly used buffers Tris-HCl (pH 8), ammonium...

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Bibliographic Details
Main Authors: Hao, Piliang, Ren, Yan, Datta, Arnab, Tam, James P., Sze, Siu Kwan
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2015
Subjects:
ERLIC
HCD
Trypsin digestion buffers
Artificial deamidation
Glycosylation
Online Access:https://hdl.handle.net/10356/81183
http://hdl.handle.net/10220/39156
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Institution: Nanyang Technological University
Language: English

Internet

https://hdl.handle.net/10356/81183
http://hdl.handle.net/10220/39156

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