Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation

• المؤلفون الرئيسيون: Hao, Piliang, Ren, Yan, Datta, Arnab, Tam, James P., Sze, Siu Kwan
• مؤلفون آخرون: School of Biological Sciences
• التنسيق: مقال
• اللغة: English
• منشور في: 2015
• الموضوعات: ERLIC; HCD; Trypsin digestion buffers; Artificial deamidation; Glycosylation
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/81183; http://hdl.handle.net/10220/39156

Nonenzymatic deamidation occurs readily under the condition of trypsin digestion, resulting in the identification of many artificial deamidation sites. To evaluate the effect of trypsin digestion buffers on artificial deamidation, we compared the three commonly used buffers Tris-HCl (pH 8), ammonium...