Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation

• Main Authors: Hao, Piliang, Ren, Yan, Datta, Arnab, Tam, James P., Sze, Siu Kwan
• 其他作者: School of Biological Sciences
• 格式: Article
• 語言: English
• 出版: 2015
• 主題: ERLIC; HCD; Trypsin digestion buffers; Artificial deamidation; Glycosylation
• 在線閱讀: https://hdl.handle.net/10356/81183; http://hdl.handle.net/10220/39156
• 機構: Nanyang Technological University

Nonenzymatic deamidation occurs readily under the condition of trypsin digestion, resulting in the identification of many artificial deamidation sites. To evaluate the effect of trypsin digestion buffers on artificial deamidation, we compared the three commonly used buffers Tris-HCl (pH 8), ammonium...