The fetal mouse metatarsal bone explant as a model of angiogenesis
The mouse fetal metatarsal provides a unique tool for studying angiogenesis. In comparison with other commonly used in vitro or ex vivo angiogenesis assays, vessel outgrowth from mouse fetal metatarsals is more representative of sprouting angiogensis in vivo. It allows the analysis of blood vessel g...
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sg-ntu-dr.10356-820992020-11-01T05:10:41Z The fetal mouse metatarsal bone explant as a model of angiogenesis Song, Weihua Fhu, Chee Wai Ang, Koon Hwee Liu, Cheng Hao Johari, Nurul Azizah Binte Lio, Daniel Abraham, Sabu Hong, Wanjin Moss, Stephen E Greenwood, John Wang, Xiaomeng Lee Kong Chian School of Medicine (LKCMedicine) Cell and Tissue Culture Functional Analysis The mouse fetal metatarsal provides a unique tool for studying angiogenesis. In comparison with other commonly used in vitro or ex vivo angiogenesis assays, vessel outgrowth from mouse fetal metatarsals is more representative of sprouting angiogensis in vivo. It allows the analysis of blood vessel growth, and the mechanisms underpinning this process, in a multicellular microenvironment that drives the formation of a robust and complex vascular network in the absence of exogenous growth factors. By labeling different constituents of the vascular structure, it is possible to perform 3D rendering of the spatial interplay between different cellular components and to carry out quantitative analysis of vessel outgrowth. High-resolution imaging permits the visualization of fine structural and cellular details. As the assay involves the use of fetal tissues, it is possible to follow new blood vessel formation in genetically modified mice that are perinatally lethal. The entire process takes 9–13 d. A detailed description of how to set up and perform the assay is described here. NMRC (Natl Medical Research Council, S’pore) Accepted version 2016-08-05T08:40:27Z 2019-12-06T14:46:33Z 2016-08-05T08:40:27Z 2019-12-06T14:46:33Z 2015 Journal Article Song, W., Fhu, C. W., Ang, K. H., Liu, C. H., Johari, N. A. B., Lio, D., et al. (2015). The fetal mouse metatarsal bone explant as a model of angiogenesis. Nature Protocols, 10(10), 1459-1473. https://hdl.handle.net/10356/82099 http://hdl.handle.net/10220/41107 10.1038/nprot.2015.097 en Nature Protocols © 2015 Macmillan Publishers Limited, part of Springer Nature. This is the author created version of a work that has been peer reviewed and accepted for publication by Nature Protocols, Macmillan Publishers Limited. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1038/nprot.2015.097]. 26 p. application/pdf |
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Cell and Tissue Culture Functional Analysis Song, Weihua Fhu, Chee Wai Ang, Koon Hwee Liu, Cheng Hao Johari, Nurul Azizah Binte Lio, Daniel Abraham, Sabu Hong, Wanjin Moss, Stephen E Greenwood, John Wang, Xiaomeng The fetal mouse metatarsal bone explant as a model of angiogenesis |
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The mouse fetal metatarsal provides a unique tool for studying angiogenesis. In comparison with other commonly used in vitro or ex vivo angiogenesis assays, vessel outgrowth from mouse fetal metatarsals is more representative of sprouting angiogensis in vivo. It allows the analysis of blood vessel growth, and the mechanisms underpinning this process, in a multicellular microenvironment that drives the formation of a robust and complex vascular network in the absence of exogenous growth factors. By labeling different constituents of the vascular structure, it is possible to perform 3D rendering of the spatial interplay between different cellular components and to carry out quantitative analysis of vessel outgrowth. High-resolution imaging permits the visualization of fine structural and cellular details. As the assay involves the use of fetal tissues, it is possible to follow new blood vessel formation in genetically modified mice that are perinatally lethal. The entire process takes 9–13 d. A detailed description of how to set up and perform the assay is described here. |
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Lee Kong Chian School of Medicine (LKCMedicine) |
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Lee Kong Chian School of Medicine (LKCMedicine) Song, Weihua Fhu, Chee Wai Ang, Koon Hwee Liu, Cheng Hao Johari, Nurul Azizah Binte Lio, Daniel Abraham, Sabu Hong, Wanjin Moss, Stephen E Greenwood, John Wang, Xiaomeng |
format |
Article |
author |
Song, Weihua Fhu, Chee Wai Ang, Koon Hwee Liu, Cheng Hao Johari, Nurul Azizah Binte Lio, Daniel Abraham, Sabu Hong, Wanjin Moss, Stephen E Greenwood, John Wang, Xiaomeng |
author_sort |
Song, Weihua |
title |
The fetal mouse metatarsal bone explant as a model of angiogenesis |
title_short |
The fetal mouse metatarsal bone explant as a model of angiogenesis |
title_full |
The fetal mouse metatarsal bone explant as a model of angiogenesis |
title_fullStr |
The fetal mouse metatarsal bone explant as a model of angiogenesis |
title_full_unstemmed |
The fetal mouse metatarsal bone explant as a model of angiogenesis |
title_sort |
fetal mouse metatarsal bone explant as a model of angiogenesis |
publishDate |
2016 |
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https://hdl.handle.net/10356/82099 http://hdl.handle.net/10220/41107 |
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1683493014804103168 |