The stimulating role of subunit F in ATPase activity inside the A1-complex of the Methanosarcina mazei Gö1 A1AO ATP synthase

• Main Authors: Singh, Dhirendra, Sielaff, Hendrik, Sundararaman, Lavanya, Bhushan, Shashi, Grüber, Gerhard
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2016
• Subjects: A1AO ATP synthase; Subunit F
• Online Access: https://hdl.handle.net/10356/82139; http://hdl.handle.net/10220/41122
• Institution: Nanyang Technological University

A1AO ATP synthases couple ion-transport of the AO sector and ATP synthesis/hydrolysis of the A3B3-headpiece via their stalk subunits D and F. Here, we produced and purified stable A3B3D- and A3B3DF-complexes of the Methanosarcina mazei Gö1 A-ATP synthase as confirmed by electron microscopy. Enzymati...