Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis
Objective: DNA methylation may be a stable epigenetic contributor to defining fat cell lineage. Methods: We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis....
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sg-ntu-dr.10356-830342022-02-16T16:31:13Z Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis Lim, Yen Ching Chia, Sook Yoong Jin, Shengnan Han, Weiping Ding, Chunming Sun, Lei Lee Kong Chian School of Medicine (LKCMedicine) Brown adipogenesis White adipogenesis Objective: DNA methylation may be a stable epigenetic contributor to defining fat cell lineage. Methods: We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis. Results: Our analysis demonstrated that DNA methylation is a stable epigenetic signature for brown and white cell lineage before, during, and after differentiation. We identified 31 genes whose promoters were significantly differentially methylated between white and brown adipogenesis at all three time points of differentiation. Among them, five genes belong to the Hox family; their expression levels were anti-correlated with promoter methylation, suggesting a regulatory role of DNA methylation in transcription. Blocking DNA methylation with 5-Aza-cytidine increased the expression of these genes, with the most prominent effect on Hoxc10, a repressor of BAT marker expression. Conclusions: Our data suggest that DNA methylation may play an important role in lineage-specific development in adipocytes. ASTAR (Agency for Sci., Tech. and Research, S’pore) NMRC (Natl Medical Research Council, S’pore) Published version 2017-05-11T06:05:20Z 2019-12-06T15:10:36Z 2017-05-11T06:05:20Z 2019-12-06T15:10:36Z 2016 Journal Article Lim, Y. C., Chia, S. Y., Jin, S., Han, W., Ding, C., & Sun, L. (2016). Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis. Molecular Metabolism, 5(10), 1033-1041. 2212-8778 https://hdl.handle.net/10356/83034 http://hdl.handle.net/10220/42372 10.1016/j.molmet.2016.08.006 27689016 en Molecular Metabolism © 2016 The Author(s). Published by Elsevier GmbH. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). 9 p. application/pdf |
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Brown adipogenesis White adipogenesis Lim, Yen Ching Chia, Sook Yoong Jin, Shengnan Han, Weiping Ding, Chunming Sun, Lei Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
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Objective: DNA methylation may be a stable epigenetic contributor to defining fat cell lineage.
Methods: We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis.
Results: Our analysis demonstrated that DNA methylation is a stable epigenetic signature for brown and white cell lineage before, during, and after differentiation. We identified 31 genes whose promoters were significantly differentially methylated between white and brown adipogenesis at all three time points of differentiation. Among them, five genes belong to the Hox family; their expression levels were anti-correlated with promoter methylation, suggesting a regulatory role of DNA methylation in transcription. Blocking DNA methylation with 5-Aza-cytidine increased the expression of these genes, with the most prominent effect on Hoxc10, a repressor of BAT marker expression.
Conclusions: Our data suggest that DNA methylation may play an important role in lineage-specific development in adipocytes. |
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Lee Kong Chian School of Medicine (LKCMedicine) |
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Lee Kong Chian School of Medicine (LKCMedicine) Lim, Yen Ching Chia, Sook Yoong Jin, Shengnan Han, Weiping Ding, Chunming Sun, Lei |
format |
Article |
author |
Lim, Yen Ching Chia, Sook Yoong Jin, Shengnan Han, Weiping Ding, Chunming Sun, Lei |
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Lim, Yen Ching |
title |
Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title_short |
Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title_full |
Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title_fullStr |
Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title_full_unstemmed |
Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
title_sort |
dynamic dna methylation landscape defines brown and white cell specificity during adipogenesis |
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2017 |
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https://hdl.handle.net/10356/83034 http://hdl.handle.net/10220/42372 |
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1725985698840838144 |