Conformational dynamics of the rotary subunit F in the A3B3DF complex of Methanosarcina mazei Gö1 A-ATP synthase monitored by single-molecule FRET

• Main Authors: Singh, Dhirendra, Sielaff, Hendrik, Börsch, Michael, Grüber, Gerhard
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2017
• Subjects: Forster resonance energy transfer; ATP synthase; Bioenergetics
• Online Access: https://hdl.handle.net/10356/83463; http://hdl.handle.net/10220/42636
• Institution: Nanyang Technological University

In archaea the A1AO ATP synthase uses a transmembrane electrochemical potential to generate ATP, while the soluble A1 domain (subunits A3B3DF) alone can hydrolyse ATP. The three nucleotide-binding AB pairs form a barrel-like structure with a central orifice that hosts the rotating central stalk subu...