Safety and feasibility of intrastromal injection of cultivated human corneal stromal keratocytes as cell-based therapy for corneal opacities

• Main Authors: Yam, Gary Hin-Fai, Fuest, Matthias, Nur Zahirah M. Yusoff, Mehta, Jodhbir Singh, Goh, Tze-Wei, Bandeira, Francisco, Setiawan, Melina, Seah, Xin-Yi, Lwin, Nyein-Chan, Stanzel, Tisha P., Ong, Hon-Shing
• Other Authors: School of Materials Science & Engineering
• Format: Article
• Language: English
• Published: 2019
• Subjects: Science::Medicine; Intrastromal Injection; Corneal Opacities
• Online Access: https://hdl.handle.net/10356/84634; http://hdl.handle.net/10220/49158
• Institution: Nanyang Technological University

Purpose: To evaluate the safety and feasibility of intrastromal injection of human corneal stromal keratocytes (CSKs) and its therapeutic effect on a rodent early corneal opacity model. Methods: Twelve research-grade donor corneas were used in primary culture to generate quiescent CSKs and activated...