Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion

Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion

Complementation-dependent fluorescence is a powerful way to study co-localization or interactions between biomolecules. A split-GFP variant, involving the self-associating GFP 1–10 and GFP 11, has previously provided a convenient approach to measure recombinant protein titers in cell supernatants. A...

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Bibliographic Details
Main Authors: Lundqvist, Magnus, Thalén, Niklas, Volk, Anna-Luisa, Hansen, Henning Gram, von Otter, Eric, Nygren, Per-Åke, Uhlen, Mathias, Rockberg, Johan
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2019
Subjects:
DRNTU::Science::Biological sciences
Chromophore
Protein Secretion
Online Access:https://hdl.handle.net/10356/85425
http://hdl.handle.net/10220/48210
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Institution: Nanyang Technological University
Language: English

Internet

https://hdl.handle.net/10356/85425
http://hdl.handle.net/10220/48210

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