Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion
Complementation-dependent fluorescence is a powerful way to study co-localization or interactions between biomolecules. A split-GFP variant, involving the self-associating GFP 1–10 and GFP 11, has previously provided a convenient approach to measure recombinant protein titers in cell supernatants. A...
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Main Authors: | , , , , , , , |
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Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2019
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Subjects: | |
Online Access: | https://hdl.handle.net/10356/85425 http://hdl.handle.net/10220/48210 |
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Institution: | Nanyang Technological University |
Language: | English |