Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells
Pluripotent stem cells have been proposed as an unlimited source of pancreatic β cells for studying and treating diabetes. However, the long, multi-step differentiation protocols used to generate functional β cells inevitably exhibit considerable variability, particularly when applied to pluripotent...
Saved in:
Main Authors: | , , , , , , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2017
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/86521 http://hdl.handle.net/10220/44039 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-86521 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-865212021-11-25T05:05:34Z Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells Trott, Jamie Tan, Ee Kim Ong, Sheena Titmarsh, Drew M. Denil, Simon L.I.J. Giam, Maybelline Wong, Cheng Kit Wang, Jiaxu Shboul, Mohammad Eio, Michelle Cooper-White, Justin Cool, Simon M. Rancati, Giulia Stanton, Lawrence W. Reversade, Bruno Dunn, Norris Ray Lee Kong Chian School of Medicine (LKCMedicine) School of Biological Sciences Culture Conditions Pancreatic Progenitors Pluripotent stem cells have been proposed as an unlimited source of pancreatic β cells for studying and treating diabetes. However, the long, multi-step differentiation protocols used to generate functional β cells inevitably exhibit considerable variability, particularly when applied to pluripotent cells from diverse genetic backgrounds. We have developed culture conditions that support long-term self-renewal of human multipotent pancreatic progenitors, which are developmentally more proximal to the specialized cells of the adult pancreas. These cultured pancreatic progenitor (cPP) cells express key pancreatic transcription factors, including PDX1 and SOX9, and exhibit transcriptomes closely related to their in vivo counterparts. Upon exposure to differentiation cues, cPP cells give rise to pancreatic endocrine, acinar, and ductal lineages, indicating multilineage potency. Furthermore, cPP cells generate insulin+ β-like cells in vitro and in vivo, suggesting that they offer a convenient alternative to pluripotent cells as a source of adult cell types for modeling pancreatic development and diabetes. ASTAR (Agency for Sci., Tech. and Research, S’pore) EDB (Economic Devt. Board, S’pore) Published version 2017-11-15T03:06:28Z 2019-12-06T16:23:54Z 2017-11-15T03:06:28Z 2019-12-06T16:23:54Z 2017 Journal Article Trott, J., Tan, E. K., Ong, S., Titmarsh, D. M., Denil, S. L., Giam, M., et al. (2017). Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells. Stem Cell Reports, 8(6), 1675-1688. 2213-6711 https://hdl.handle.net/10356/86521 http://hdl.handle.net/10220/44039 10.1016/j.stemcr.2017.05.019 en Stem Cell Reports © 2017 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). 14 p. application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
Culture Conditions Pancreatic Progenitors |
spellingShingle |
Culture Conditions Pancreatic Progenitors Trott, Jamie Tan, Ee Kim Ong, Sheena Titmarsh, Drew M. Denil, Simon L.I.J. Giam, Maybelline Wong, Cheng Kit Wang, Jiaxu Shboul, Mohammad Eio, Michelle Cooper-White, Justin Cool, Simon M. Rancati, Giulia Stanton, Lawrence W. Reversade, Bruno Dunn, Norris Ray Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells |
description |
Pluripotent stem cells have been proposed as an unlimited source of pancreatic β cells for studying and treating diabetes. However, the long, multi-step differentiation protocols used to generate functional β cells inevitably exhibit considerable variability, particularly when applied to pluripotent cells from diverse genetic backgrounds. We have developed culture conditions that support long-term self-renewal of human multipotent pancreatic progenitors, which are developmentally more proximal to the specialized cells of the adult pancreas. These cultured pancreatic progenitor (cPP) cells express key pancreatic transcription factors, including PDX1 and SOX9, and exhibit transcriptomes closely related to their in vivo counterparts. Upon exposure to differentiation cues, cPP cells give rise to pancreatic endocrine, acinar, and ductal lineages, indicating multilineage potency. Furthermore, cPP cells generate insulin+ β-like cells in vitro and in vivo, suggesting that they offer a convenient alternative to pluripotent cells as a source of adult cell types for modeling pancreatic development and diabetes. |
author2 |
Lee Kong Chian School of Medicine (LKCMedicine) |
author_facet |
Lee Kong Chian School of Medicine (LKCMedicine) Trott, Jamie Tan, Ee Kim Ong, Sheena Titmarsh, Drew M. Denil, Simon L.I.J. Giam, Maybelline Wong, Cheng Kit Wang, Jiaxu Shboul, Mohammad Eio, Michelle Cooper-White, Justin Cool, Simon M. Rancati, Giulia Stanton, Lawrence W. Reversade, Bruno Dunn, Norris Ray |
format |
Article |
author |
Trott, Jamie Tan, Ee Kim Ong, Sheena Titmarsh, Drew M. Denil, Simon L.I.J. Giam, Maybelline Wong, Cheng Kit Wang, Jiaxu Shboul, Mohammad Eio, Michelle Cooper-White, Justin Cool, Simon M. Rancati, Giulia Stanton, Lawrence W. Reversade, Bruno Dunn, Norris Ray |
author_sort |
Trott, Jamie |
title |
Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells |
title_short |
Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells |
title_full |
Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells |
title_fullStr |
Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells |
title_full_unstemmed |
Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells |
title_sort |
long-term culture of self-renewing pancreatic progenitors derived from human pluripotent stem cells |
publishDate |
2017 |
url |
https://hdl.handle.net/10356/86521 http://hdl.handle.net/10220/44039 |
_version_ |
1718368094274977792 |