A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation

• Main Authors: Hou, Wen, Liu, Lei, Zhang, Xiaohong, Liu, Chuanfa
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2019
• Subjects: Science::Biological sciences; Sequential Peptide Ligation; Thioacid Capture Ligation
• Online Access: https://hdl.handle.net/10356/87104; http://hdl.handle.net/10220/49869
• Institution: Nanyang Technological University

Sequential peptide ligation strategy becomes more and more important in large protein or long peptides chemical synthesis due to the limited peptide/protein size obtained by solid phase synthesis of individual peptides or even one-step peptide ligation. Herein, we developed an alternative method which could perform the sequential peptide ligation of several segments from N to C direction based on the combined use of thioacid capture ligation and native chemical ligation. The sweet protein monellin was produced through this strategy on a scale of multi-milligrams.