Consensus design and engineering of an efficient and high-yield peptide asparaginyl ligase for protein cyclization and ligation

• Main Authors: Hemu, Xinya, Zhang, Xiaohong, Chang, Hong Yi, En, Poh Jin, Tam, James P.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2023
• Subjects: Science::Biological sciences; Peptide Asparaginyl Ligase; Asparaginyl Endopeptidase; Plant Legumain; Consensus Design; Cyclization; Ligation
• Online Access: https://hdl.handle.net/10356/164805
• Institution: Nanyang Technological University

Plant legumains are Asn/Asp-specific endopeptidases (AEPs) that have diverse functions in plants. Peptide asparaginyl ligases (PALs) are a special legumain subtype that primarily catalyze peptide bond formation rather than hydrolysis. PALs are versatile protein engineering tools but are rarely fo...