Ligase-controlled cyclo-oligomerization of peptides

by Hemu, Xinya, Zhang, Xiaohong, Tam, James P.
Published 2020

Immobilized peptide asparaginyl ligases enhance stability and facilitate macrocyclization and site-specific ligation

by Hemu, Xinya, To, Janet, Zhang, Xiaohong, Tam, James P.
Published 2021

A thioethylalkylamido (TEA) thioester surrogate in the synthesis of a cyclic peptide via a tandem acyl shift

by Taichi, Misako, Hemu, Xinya, Qiu, Yibo, Tam, James P.
Published 2013

Selective bi-directional amide bond cleavage of N-methylcysteinyl peptide

by Qiu, Yibo, Hemu, Xinya, Liu, Ding Xiang, Tam, James P.
Published 2014

Biomimetic synthesis of cyclic peptides using novel thioester surrogates

by Hemu, Xinya, Taichi, Misako, Qiu, Yibo, Liu, Ding-Xiang, Tam, James P.
Published 2016

Consensus design and engineering of an efficient and high-yield peptide asparaginyl ligase for protein cyclization and ligation

by Hemu, Xinya, Zhang, Xiaohong, Chang, Hong Yi, En, Poh Jin, Tam, James P.
Published 2023

Immobilization and intracellular delivery of circular proteins by modifying a genetically incorporated unnatural amino acid

by Bi, Xiaobao, Yin, Juan, Hemu, Xinya, Rao, Chang, Tam, James P., Liu, Chuan-Fa
Published 2020

Butelase 1 is an Asx-specific ligase enabling peptide macrocyclization and synthesis

by Qiu, Yibo, Lian, Yilong, Hemu, Xinya, Wang, Shujing, Nguyen, Giang Kien Truc, Tam, James Pingkwan
Published 2015

Butelase-mediated cyclization and ligation of peptides and proteins

by Qiu, Yibo, Nguyen, Giang Kien Truc, Cao, Yuan, Hemu, Xinya, Liu, Chuan-Fa, Tam, James Pingkwan
Published 2016

A high-throughput peptidomic strategy to decipher the molecular diversity of cyclic cysteine-rich peptides

by Serra, Aida, Hemu, Xinya, Nguyen, Ngan T. K., Sze, Siu Kwan, Tam, James P., Nguyen, Giang Kien Truc
Published 2016

Commercial processed soy-based food product contains glycated and glycoxidated lunasin proteoforms

by Serra, Aida, Gallart-Palau, Xavier, See-Toh, Rachel Su-En, Hemu, Xinya, Sze, Siu Kwan, Tam, James Pingkwan
Published 2018

Engineering protein theranostics using bio-orthogonal asparaginyl peptide ligases

by Wang, Zhen, Zhang, Dingpeng, Hemu, Xinya, Hu, Side, To, Janet, Zhang, Xiaohong, Lescar, Julien, Tam, James P., Liu, Chuan-Fa
Published 2021

Characterization and application of natural and recombinant butelase-1 to improve industrial enzymes by end-to-end circularization

by Hemu, Xinya, Zhang, Xiaohong, Nguyen, Giang K. T., To, Janet, Serra, Aida, Loo, Shining, Sze, Siu Kwan, Liu, Chuan-Fa, Tam, James P.
Published 2022

The legumain McPAL1 from Momordica cochinchinensis is a highly stable Asx-specific splicing enzyme

by Liew, Heng Tai, To, Janet, Zhang, Xiaohong, Hemu, Xinya, Chan, Ning-Yu, Serra, Aida, Sze, Siu Kwan, Liu, Chuan-Fa, Tam, James P.
Published 2022

Substrate-binding glycine residues are major determinants for hydrolase and ligase activity of plant legumains

by Hemu, Xinya, Chan, Ning-Yu, Liew, Heng Tai, Hu, Side, Zhang, Xiaohong, Serra, Aida, Lescar, Julien, Liu, Chuan-Fa, Tam, James P.
Published 2023

Turning an asparaginyl endopeptidase into a peptide ligase

by Hemu, Xinya, El Sahili, Abbas, Hu, Side, Zhang, Xiaohong, Serra, Aida, Goh, Boon Chong, Darwis, Dina Amallia, Chen, Ming Wei, Sze, Siu Kwan, Liu, Chuan-Fa, Lescar, Julien, Tam, James P.
Published 2021

Structural determinants for peptide-bond formation by asparaginyl ligases

by Hemu, Xinya, El Sahili, Abbas, Hu, Side, Wong, Kaho, Chen, Yu, Wong, Yee Hwa, Zhang, Xiaohong, Serra, Aida, Goh, Boon Chong, Darwis, Dina Amallia, Chen, Ming Wei, Sze, Siu Kwan, Liu, Chuan-Fa, Lescar, Julien, Tam, James P.
Published 2021