Characterization and application of natural and recombinant butelase-1 to improve industrial enzymes by end-to-end circularization

• Main Authors: Hemu, Xinya, Zhang, Xiaohong, Nguyen, Giang K. T., To, Janet, Serra, Aida, Loo, Shining, Sze, Siu Kwan, Liu, Chuan-Fa, Tam, James P.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2022
• Subjects: Science::Biological sciences; Unprotected Peptide Segments; Cyclic-Peptides
• Online Access: https://hdl.handle.net/10356/160046
• Institution: Nanyang Technological University

Butelase-1, an asparaginyl endopeptidase or legumain, is the prototypical and fastest known Asn/Asp-specific peptide ligase. It is highly useful for engineering and macrocyclization of peptides and proteins. However, certain biochemical properties and applications of naturally occurring and recombin...