Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses to Nippostrongylus brasiliensis

The induction of Th2 responses is thought to be multifactorial, and emerge from specific pathways distinct from those associated with antagonistic antibacterial or antiviral Th1 responses. Here, we show that the recognition of non-viable Nippostrongylus brasiliensis (Nb) in the skin induces a strong...

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Main Authors: Pellefigues, Christophe, Tang, Shiau-Choot, Schmidt, Alfonso, White, Ruby F., Lamiable, Olivier, Connor, Lisa M., Ruedl, Christiane, Dobrucki, Jurek, Le Gros, Graham, Ronchese, Franca
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2018
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Online Access:https://hdl.handle.net/10356/87377
http://hdl.handle.net/10220/44458
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-873772023-02-28T17:01:12Z Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses to Nippostrongylus brasiliensis Pellefigues, Christophe Tang, Shiau-Choot Schmidt, Alfonso White, Ruby F. Lamiable, Olivier Connor, Lisa M. Ruedl, Christiane Dobrucki, Jurek Le Gros, Graham Ronchese, Franca School of Biological Sciences Helminth Nippostrongylus Brasiliensis The induction of Th2 responses is thought to be multifactorial, and emerge from specific pathways distinct from those associated with antagonistic antibacterial or antiviral Th1 responses. Here, we show that the recognition of non-viable Nippostrongylus brasiliensis (Nb) in the skin induces a strong recruitment of monocytes and neutrophils and the release of neutrophil extracellular traps (NETs). Nb also activates toll-like receptor 4 (TLR4) signaling with expression of Ifnb transcripts in the skin and the development of an IFN type I signature on helminth antigen-bearing dendritic cells in draining lymph nodes. Co-injection of Nb together with about 10,000 Gram-negative bacteria amplified this TLR4-dependent but NET-independent IFN type I response and enhanced the development of Th2 responses. Thus, a limited activation of antibacterial signaling pathways is able to boost antihelminthic responses, suggesting a role for bacterial sensing in the optimal induction of Th2 immunity. Published version 2018-02-28T04:16:07Z 2019-12-06T16:40:34Z 2018-02-28T04:16:07Z 2019-12-06T16:40:34Z 2017 Journal Article Pellefigues, C., Tang, S.-C., Schmidt, A., White, R. F., Lamiable, O., Connor, L. M., et al. (2017). Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses to Nippostrongylus brasiliensis. Frontiers in Immunology, 8, 1575-. https://hdl.handle.net/10356/87377 http://hdl.handle.net/10220/44458 10.3389/fimmu.2017.01575 en Frontiers in Immunology © 2017 Pellefigues, Tang, Schmidt, White, Lamiable, Connor, Ruedl, Dobrucki, Le Gros and Ronchese. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 14 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Helminth
Nippostrongylus Brasiliensis
spellingShingle Helminth
Nippostrongylus Brasiliensis
Pellefigues, Christophe
Tang, Shiau-Choot
Schmidt, Alfonso
White, Ruby F.
Lamiable, Olivier
Connor, Lisa M.
Ruedl, Christiane
Dobrucki, Jurek
Le Gros, Graham
Ronchese, Franca
Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses to Nippostrongylus brasiliensis
description The induction of Th2 responses is thought to be multifactorial, and emerge from specific pathways distinct from those associated with antagonistic antibacterial or antiviral Th1 responses. Here, we show that the recognition of non-viable Nippostrongylus brasiliensis (Nb) in the skin induces a strong recruitment of monocytes and neutrophils and the release of neutrophil extracellular traps (NETs). Nb also activates toll-like receptor 4 (TLR4) signaling with expression of Ifnb transcripts in the skin and the development of an IFN type I signature on helminth antigen-bearing dendritic cells in draining lymph nodes. Co-injection of Nb together with about 10,000 Gram-negative bacteria amplified this TLR4-dependent but NET-independent IFN type I response and enhanced the development of Th2 responses. Thus, a limited activation of antibacterial signaling pathways is able to boost antihelminthic responses, suggesting a role for bacterial sensing in the optimal induction of Th2 immunity.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Pellefigues, Christophe
Tang, Shiau-Choot
Schmidt, Alfonso
White, Ruby F.
Lamiable, Olivier
Connor, Lisa M.
Ruedl, Christiane
Dobrucki, Jurek
Le Gros, Graham
Ronchese, Franca
format Article
author Pellefigues, Christophe
Tang, Shiau-Choot
Schmidt, Alfonso
White, Ruby F.
Lamiable, Olivier
Connor, Lisa M.
Ruedl, Christiane
Dobrucki, Jurek
Le Gros, Graham
Ronchese, Franca
author_sort Pellefigues, Christophe
title Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses to Nippostrongylus brasiliensis
title_short Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses to Nippostrongylus brasiliensis
title_full Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses to Nippostrongylus brasiliensis
title_fullStr Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses to Nippostrongylus brasiliensis
title_full_unstemmed Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses to Nippostrongylus brasiliensis
title_sort toll-like receptor 4, but not neutrophil extracellular traps, promote ifn type i expression to enhance th2 responses to nippostrongylus brasiliensis
publishDate 2018
url https://hdl.handle.net/10356/87377
http://hdl.handle.net/10220/44458
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