Real Time, Spatial, and Temporal Mapping of the Distribution of c-di-GMP during Biofilm Development

• Main Authors: Nair, Harikrishnan A. S., Periasamy, Saravanan, Yang, Liang, Kjelleberg, Staffan, Rice, Scott A.
• Other Authors: Interdisciplinary Graduate School (IGS)
• Format: Article
• Language: English
• Published: 2018
• Subjects: Bacteria; Cyclic di-GMP (c-di-GMP)
• Online Access: https://hdl.handle.net/10356/87406; http://hdl.handle.net/10220/44416
• Institution: Nanyang Technological University

Bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) is a dynamic intracellular signaling molecule that plays a central role in the biofilm life cycle. Current methodologies for the quantification of c-di-GMP are typically based on chemical extraction, representing end point measurements. C...